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SMURF2 antibody (AA 601-700)

Name: SMURF2 antibody (AA 601-700)
SKU: ABIN753178
Price: 384.62 USD
Availability: InStock

SMURF2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse embryo lysates probed with Rabbit Anti-SMURF2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-SMURF2 Polyclonal Antibody, Unconjugated (ABIN753178) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SMURF2 Polyclonal Antibody, Unconjugated (ABIN753178) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining

IHC

Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-SMURF2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

(1 reference)

Catalog No. ABIN753178

Datasheet as PDF

Target See all SMURF2 Antibodies

SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
Binding Specificity
15

9

8

7

7

6

5

2

2

1

1

1

1
AA 601-700

Reactivity

Human, Mouse, Rat
Host
62

3
Rabbit
Compatible Secondaries
Clonality
55

10
Polyclonal
Conjugate
19

7

7

6

5

4

2

2

2

2

2

2

1

1

1

1

1
This SMURF2 antibody is un-conjugated
Application
53

33

28

17

13

12

12

12

7

2

2
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Horse,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human SMURF2

Isotype

IgG
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibody
SMURF2 Reactivity: Human, Rat WB, IF, IHC (p) Host: Rabbit Polyclonal RB4107-4108 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731) antibody
SMURF2 Reactivity: Human, Mouse WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) antibody
SMURF2 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (Internal Region) antibody
SMURF2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody
SMURF2 Reactivity: Human WB Host: Mouse Monoclonal 1826CT865-51-28 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 150-250) antibody
SMURF2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (C-Term) antibody
SMURF2 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody
SMURF2 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) antibody (HRP)
SMURF2 Reactivity: Human ELISA Host: Rabbit Polyclonal HRP

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) antibody (FITC)
SMURF2 Reactivity: Human Host: Rabbit Polyclonal FITC

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Huang, Yang, Nan, Gao, Feng, Gou, Chen, Zhang, Yan, Peng, Xu: "The proteasome inhibitor, MG132, attenuates diabetic nephropathy by inhibiting SnoN degradation in vivo and in vitro." in: BioMed research international, Vol. 2014, pp. 684765, (2015) (PubMed).
Target

SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Alternative Name

SMURF2 (SMURF2 Products)

Background

Synonyms: E3 ubiquitin-protein ligase SMURF2, hSMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2
Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Gene ID

64750

UniProt

Q9HAU4