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SIAH1 antibody (N-Term)

Name: SIAH1 antibody (N-Term)
Brand: antibodies-online
SKU: ABIN184675
Price: 469.28 USD
Availability: InStock

Verified SIAH1 Reactivity: Human, Rat WB, ELISA, IF Host: Goat Polyclonal unconjugated

(ABIN184675) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.

ABIN184675 (1µg/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35µg protein in RIPA buffer).

ABIN184675 staining (1µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).

(ABIN184675) (1 μg/mL) staining of Human Liver lysate (35 μg protein in RIPA buffer).

(ABIN184675) (1 μg/mL) staining of RatLiver lysate (35 μg protein in RIPA buffer).

(ABIN184675) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.

8 images

(1 reference)

Catalog No. ABIN184675

Datasheet as PDF

Target See all SIAH1 Antibodies

SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
Binding Specificity
15

7

7

6

6

5

2

2

1

1

1

1
N-Term
Reactivity
36

24

24

5

5

4

4

4

3

2

2

2

2

2

2
Human, Rat
Host
48

2

1
Goat
Compatible Secondaries
Clonality
49

2
Polyclonal
Conjugate
19

5

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1
This SIAH1 antibody is un-conjugated
Application
42

22

13

13

6

5

3

3

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose

SIAH1

Sequence

SRQTATALPT GTSKC

Specificity

This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)

Cross-Reactivity

Cow, Dog, Human, Mouse, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.

Isotype

IgG
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibody
SIAH1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibody
SIAH1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C5 unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
SIAH1 Reactivity: Human, Rat, Mouse, Dog, Cow, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
SIAH1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 191-240) antibody
SIAH1 Reactivity: Human, Rat, Mouse, Dog, Cow, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit, Xenopus laevis, Chicken, Pig, Bat, Hamster, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (C-Term) antibody
SIAH1 Reactivity: Human, Rat, Mouse, Dog, Cow, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibody
SIAH1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 201-282) antibody
SIAH1 Reactivity: Rat, Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody (Biotin)
Verified SIAH1 Reactivity: Human, Rat, Mouse WB, ELISA, IP Host: Goat Polyclonal Biotin

anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibody
SIAH1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2F26 unconjugated

Application Notes

Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336
Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Sroka, Voigt, Deeg, Reed, Schulz, Bhr, Kermer: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).
Target

SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name

SIAH1 (SIAH1 Products)

Synonyms

SIAH antibody, Siah1 antibody, Siah1a antibody, id:ibd2635 antibody, zgc:56026 antibody, SIAH1A antibody, siah antibody, E3 ubiquitin-protein ligase Siah1 antibody, E3 ubiquitin-protein ligase siah-1 antibody, siah E3 ubiquitin protein ligase 1 antibody, siah E3 ubiquitin protein ligase 1 L homeolog antibody, Tsp_03055 antibody, siah-1 antibody, Siah1 antibody, siah1 antibody, SIAH1 antibody, siah1.L antibody

Background

SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog

Molecular Weight

31.1kDa

Gene ID

6477, 20437, 140941

NCBI Accession

NP_003022, NP_001006611