BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1) antibody
KO Validated
BMI1
Reactivity: Human
WB, ICC, IF, IHC (p), IP
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Cross-Reactivity
- Cat, Dog, Human, Mouse
- Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.
- Isotype
- IgG
-
- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
-
Positive Control: THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1 , 293T , A549 , H1299 , HCT116 Validation: KO/KD, Orthogonal
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)
- Alternative Name
- BMI1 proto-oncogene, polycomb ring finger
- Synonyms
- FLVI2/BMI1 antibody, PCGF4 antibody, RNF51 antibody, BMI1 proto-oncogene, polycomb ring finger antibody, BMI1 antibody
- Background
-
Synonyms: BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1
Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
- Molecular Weight
- 37 kDa
- Gene ID
- 648
- UniProt
- P35226
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