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STIM1 antibody (AA 24-685)

Name: STIM1 antibody (AA 24-685)
SKU: ABIN563045
Price: 611.43 USD
Availability: InStock

STIM1 Reactivity: Human WB, ELISA, IHC (p), IF Host: Mouse Monoclonal 5A2 unconjugated

STIM1 monoclonal antibody (M01), clone 5A2.

Immunofluorescence of monoclonal antibody to STIM1 on HeLa cell.

Detection limit for recombinant GST tagged STIM1 is approximately 0.

ELISA

Western Blot detection against Immunogen (98.

Immunoperoxidase of monoclonal antibody to STIM1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B.

ISt

5 images

(20 references)

Catalog No. ABIN563045

Datasheet as PDF

Target See all STIM1 Antibodies

STIM1 (Stromal Interaction Molecule 1 (STIM1))
Binding Specificity
16

15

15

6

6

5

5

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 24-685
Reactivity
74

57

27

5

5

2

2

2

1

1

1

1
Human
Host
79

8

1

1
Mouse
Compatible Secondaries
Clonality
72

17
Monoclonal
Conjugate
41

6

5

4

3

3

3

3

3

3

2

2

2

2

2

2

2

1
This STIM1 antibody is un-conjugated
Application
45

30

26

26

26

19

12

10

9

6

3

3

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purpose

Mouse monoclonal antibody raised against a full length recombinant STIM1.

Sequence

SHSHSEKATG TSSGANSEES TAAEFCRIDK PLCHSEDEKL SFEAVRNIHK LMDDDANGDV DVEESDEFLR EDLNYHDPTV KHSTFHGEDK LISVEDLWKA WKSSEVYNWT VDEVVQWLIT YVELPQYEET FRKLQLSGHA MPRLAVTNTT MTGAVLKMTD RSHRQKLQLK ALDTVLFGPP LLTRHNHLKD FMLVVSIVIG VGGCWFAYIQ NRYSKEHMKK MMKDLEGLHR AEQSLHDLQE RLHKAQEEHR TVEVEKVHLE KKLRDEINLA KQEAQRLKEL REGTENERSR QKYAEEELEQ VREALRKAEK ELESHSSWYA PEALQKWLQL THEVEVQYYN IKKQNAEKQL LVAKEGAEKI KKKRNTLFGT FHVAHSSSLD DVDHKILTAK QALSEVTAAL RERLHRWQQI EILCGFQIVN NPGIHSLVAA LNIDPSWMGS TRPNPAHFIM TDDVDDMDEE IVSPLSMQSP SLQSSVRQRL TEPQHGLGSQ RDLTHSDSES SLHMSDRQRV APKPPQMSRA ADEALNAMTS NGSHRLIEGV HPGSLVEKLP DSPALAKKAL LALNHGLDKA HSLMELSPSA PPGGSPHLDS SRSHSPSSPD PDTPSPVGDS RALQASRNTR IPHLAGKKAV AEEDNGSIGE ETDSSPGRKK FPLKIFKKPL KK

Cross-Reactivity

Human, Rat

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

STIM1 (AAH21300, 24 a.a. ~ 685 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

5A2

Isotype

IgG2a
anti-Stromal Interaction Molecule 1 (STIM1) (AA 1-211) antibody
STIM1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody
STIM1 Reactivity: Human, Rat, Mouse WB, IP, IHC (p), ICC Host: Mouse Monoclonal CDN3H4 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibody
STIM1 Reactivity: Human WB, IHC (p), IF, EIA Host: Mouse Monoclonal 5A2 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538), (C-Term) antibody
STIM1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB19222 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody
STIM1 Reactivity: Human, Rat, Mouse WB, IP, IHC (p), IF, ICC Host: Mouse Monoclonal CDN3H4 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibody
STIM1 Reactivity: Human WB, ELISA, IHC, IHC (p), IF Host: Mouse Monoclonal 5A2 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (AA 45-74), (N-Term) antibody
STIM1 Reactivity: Human, Rat, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (AA 56-69), (Extracellular), (N-Term) antibody
STIM1 Reactivity: Human, Rat, Mouse WB, IHC, IP, IF, ICC, FACS, LCI Host: Rabbit Polyclonal unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody
STIM1 Reactivity: Human, Rat, Mouse WB, IHC, IP, IHC (p), ICC, Inhibition Host: Mouse Monoclonal CDN3H4 unconjugated

anti-Stromal Interaction Molecule 1 (STIM1) (Middle Region) antibody
STIM1 Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse WB Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Hong, Chang, Wang, Yu, Lee: "IL-9 induces IL-8 production via STIM1 activation and ERK phosphorylation in epidermal keratinocytes: A plausible mechanism of IL-9R in atopic dermatitis." in: Journal of dermatological science, Vol. 78, Issue 3, pp. 206-14, (2015) (PubMed).

Umemura, Baljinnyam, Feske, De Lorenzo, Xie, Feng, Oda, Makino, Fujita, Yokoyama, Iwatsubo, Chen, Goydos, Ishikawa, Iwatsubo: "Store-operated Ca2+ entry (SOCE) regulates melanoma proliferation and cell migration." in: PLoS ONE, Vol. 9, Issue 2, pp. e89292, (2014) (PubMed).

Ferdaus, Xiao, Ohara, Nemoto, Harada, Saar, Hübner, Isomura, Nabika: "Identification of Stim1 as a candidate gene for exaggerated sympathetic response to stress in the stroke-prone spontaneously hypertensive rat." in: PLoS ONE, Vol. 9, Issue 4, pp. e95091, (2014) (PubMed).

Jans, Mottram, Johnson, Brown, Sikkink, Ross, Reynolds: "Lysophosphatidic acid promotes cell migration through STIM1- and Orai1-mediated Ca2+(i) mobilization and NFAT2 activation." in: The Journal of investigative dermatology, Vol. 133, Issue 3, pp. 793-802, (2013) (PubMed).

Eylenstein, Schmidt, Gu, Yang, Schmid, Schmidt, Alesutan, Szteyn, Regel, Shumilina, Lang: "Transcription factor NF-?B regulates expression of pore-forming Ca2+ channel unit, Orai1, and its activator, STIM1, to control Ca2+ entry and affect cellular functions." in: The Journal of biological chemistry, Vol. 287, Issue 4, pp. 2719-30, (2012) (PubMed).

Elvers, Herrmann, Seizer, Münzer, Beck, Schönberger, Borst, Martin-Romero, Lang, May, Gawaz: "Intracellular cyclophilin A is an important Ca(2+) regulator in platelets and critically involved in arterial thrombus formation." in: Blood, Vol. 120, Issue 6, pp. 1317-26, (2012) (PubMed).

Henke, Albrecht, Pfeiffer, Toutzaris, Zanger, Methner: "Stromal interaction molecule 1 (STIM1) is involved in the regulation of mitochondrial shape and bioenergetics and plays a role in oxidative stress." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42042-52, (2012) (PubMed).

Schmid, Bhandaru, Nurbaeva, Yang, Szteyn, Russo, Leibrock, Tyan, Pearce, Shumilina, Lang: "SGK3 regulates Ca(2+) entry and migration of dendritic cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 30, Issue 6, pp. 1423-35, (2012) (PubMed).

Kiviluoto, Decuypere, De Smedt, Missiaen, Parys, Bultynck: "STIM1 as a key regulator for Ca2+ homeostasis in skeletal-muscle development and function." in: Skeletal muscle, Vol. 1, Issue 1, pp. 16, (2011) (PubMed).

Steinbeck, Henke, Opatz, Gruszczynska-Biegala, Schneider, Theiss, Hamacher, Steinfarz, Golz, Brüstle, Kuznicki, Methner: "Store-operated calcium entry modulates neuronal network activity in a model of chronic epilepsy." in: Experimental neurology, Vol. 232, Issue 2, pp. 185-94, (2011) (PubMed).

Schuhmann, Stegner, Berna-Erro, Bittner, Braun, Kleinschnitz, Stoll, Wiendl, Meuth, Nieswandt: "Stromal interaction molecules 1 and 2 are key regulators of autoreactive T cell activation in murine autoimmune central nervous system inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 3, pp. 1536-42, (2010) (PubMed).

Saitoh, Oritani, Saito, Yokota, Ichii, Sudo, Fujita, Nakajima, Okada, Kanakura: "Identification of functional domains and novel binding partners of STIM proteins." in: Journal of cellular biochemistry, Vol. 112, Issue 1, pp. 147-56, (2010) (PubMed).

Braun, Gessner, Varga-Szabo, Syed, Konrad, Stegner, Vögtle, Schmidt, Nieswandt: "STIM1 is essential for Fcgamma receptor activation and autoimmune inflammation." in: Blood, Vol. 113, Issue 5, pp. 1097-104, (2009) (PubMed).

Wu, Wang, Young, Lai, Li: "Proteome-wide identification of novel binding partners to the oncogenic fusion gene protein, NPM-ALK, using tandem affinity purification and mass spectrometry." in: The American journal of pathology, Vol. 174, Issue 2, pp. 361-70, (2009) (PubMed).

Beyersdorf, Braun, Vögtle, Varga-Szabo, Galdos, Kissler, Kerkau, Nieswandt: "STIM1-independent T cell development and effector function in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3390-7, (2009) (PubMed).

Pani, Ong, Brazer, Liu, Rauser, Singh, Ambudkar: "Activation of TRPC1 by STIM1 in ER-PM microdomains involves release of the channel from its scaffold caveolin-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 47, pp. 20087-92, (2009) (PubMed).

Alicia, Angélica, Carlos, Alfonso, Vaca: "STIM1 converts TRPC1 from a receptor-operated to a store-operated channel: moving TRPC1 in and out of lipid rafts." in: Cell calcium, Vol. 44, Issue 5, pp. 479-91, (2008) (PubMed).

Pani, Ong, Liu, Rauser, Ambudkar, Singh: "Lipid rafts determine clustering of STIM1 in endoplasmic reticulum-plasma membrane junctions and regulation of store-operated Ca2+ entry (SOCE)." in: The Journal of biological chemistry, Vol. 283, Issue 25, pp. 17333-40, (2008) (PubMed).

Varga-Szabo, Braun, Kleinschnitz, Bender, Pleines, Pham, Renné, Stoll, Nieswandt: "The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction." in: The Journal of experimental medicine, Vol. 205, Issue 7, pp. 1583-91, (2008) (PubMed).

Grosse, Braun, Varga-Szabo, Beyersdorf, Schneider, Zeitlmann, Hanke, Schropp, Mühlstedt, Zorn, Huber, Schmittwolf, Jagla, Yu, Kerkau, Schulze, Nehls, Nieswandt: "An EF hand mutation in Stim1 causes premature platelet activation and bleeding in mice." in: The Journal of clinical investigation, Vol. 117, Issue 11, pp. 3540-50, (2007) (PubMed).

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Target

STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name

STIM1 (STIM1 Products)

Synonyms

stim1 antibody, MGC76045 antibody, STIM1 antibody, si:dkey-24p1.5 antibody, MGC154748 antibody, si:ch211-112l17.2 antibody, Stim1 antibody, D11S4896E antibody, GOK antibody, TAM antibody, SIM antibody, stromal interaction molecule 1 antibody, stromal interaction molecule 1a antibody, stromal interaction molecule 1 L homeolog antibody, stromal interaction molecule 1b antibody, STIM1 antibody, stim1 antibody, stim1a antibody, stim1.L antibody, stim1b antibody, Stim1 antibody

Background

Full Gene Name: stromal interaction molecule 1
Synonyms: D11S4896E,GOK

Gene ID

6786

Pathways

TCR Signaling, BCR Signaling