TNFAIP3 antibody (Internal Region)
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- Target See all TNFAIP3 Antibodies
- TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TNFAIP3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- A20/TNFAIP3 Antibody detects endogenous levels of total A20/TNFAIP3.
- Predicted Reactivity
- Pig,Bovine,Horse,Rabbit,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human A20/TNFAIP3, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product TNFAIP3 Primary Antibody
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anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 1-190) antibody
TNFAIP3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 1-300) antibodyTNFAIP3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 97-183) antibodyTNFAIP3 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 51-150) antibodyTNFAIP3 Reactivity: Human, Mouse, Rat ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibodyTNFAIP3 Reactivity: Human, Mouse, Rat WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) antibodyTNFAIP3 Reactivity: Human WB, IP Host: Mouse Monoclonal 59A426 unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 1-190) antibodyTNFAIP3 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (AA 321-370) antibodyTNFAIP3 Reactivity: Human, Mouse WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))
- Alternative Name
- TNFAIP3 (TNFAIP3 Products)
- Synonyms
- tnfaip3 antibody, MGC82339 antibody, TNFAIP3 antibody, A20 antibody, OTUD7C antibody, TNFA1P2 antibody, Tnfip3 antibody, TNF alpha induced protein 3 antibody, TNF alpha induced protein 3 S homeolog antibody, tumor necrosis factor, alpha-induced protein 3 antibody, TNFAIP3 antibody, tnfaip3.S antibody, Tnfaip3 antibody
- Background
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Description: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways, affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG, the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin, polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Gene: TNFAIP3
- Molecular Weight
- 90kDa
- Gene ID
- 7128
- UniProt
- P21580
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response
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