ANKIB1 Protein (AA 2-1085) (His tag)
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- Target See all ANKIB1 products
- ANKIB1 (Ankyrin Repeat and IBR Domain Containing 1 (ANKIB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-1085
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This ANKIB1 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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GNTTTKFRKA LINGDENLAC QIYENNPQLK ESLDPNISYG EPYQHNTPLH YAARHGMNRI LGTFLFGRDG NPNKRNVHNE TSMHLLCMGP QIMISEGTLH PRLARPVEDD FRRADCLQMI LQWKGAKLDQ GEYERAAIDA VDNKKNTPLH YAAASGMKAC VELLVKHGGD LFAENENRDT PCDCAEKQQH KDLALSLESQ MVFSRDPEAE EIEAEYAALD KREPYEGLRP QDLRRLKDML IVETADMLQA PLFTAEALLR AHDWDREKLL EAWMSNPENC CQRSGVQMPT PPPSGYNAWD TLPSPRTPRT TRSSVTSPDE ISLSPGDLDT SLCDICMCSI SVFEDPVDMP CGHDFCRGCW EAFLNLKIQE GEAHNIFCPA YECFQLVPVD VIESVVSKEM DKRYLQFDIK AFVENNPAIK WCPTAGCERA VRLTKQGSNP SGSDTLSFPL LRAPAVDCGK GHLFCWECLG EAHEPCDCQT WKNWLQKITE MKPEELVGVS EAYEDAANCL WLLTNSKPCA NCKSPIQKNE GCNHMQCAKC KYDFCWICLE EWKKHSSSTG GYYRCTRYEV IQHVEEQSKE MTVEAEKKHK RFQELDRFMH YYTRYKNHEH SYQLEQRLLK TAKEKMEQLS RALKETEGGC PDTTFIEDAV HVLLKTRRIL KCSYPYGFFL EPKSTKKEIF ELMQTDLEMV TEDLAQKVNR PYLRTPRHKI IRAACLVQQK RQEFLASVAR GVAPADSPDA PRRSFAGGTW DWEYLGFASP EYRRRHRQQR RRGDVHSLLS NPTDLDEPSE STFDLPEGSS GRRPGASVVS SASMSVLHSS SLRDYSPASR SANQDSLQAL SSLDEDDPNI LLAIQLSLQE SGLDMDEETR DFLSNETSLG AIGSSLPSRL DSVPRSTESP RAALSSSELL ELGDSLMRLG ADSDPFSTDT LSSRPLSETR SDFCPSSSDL DSAGQDPSAN DNLLGNIMAW FHDMNPQSIA LIPPAATTEI SAEPQLPCIR DGSEGVRDME LVPPEDSVSK DTGVHEGERA QMEENPLEEN ILAREELSQA GDSSNEAVGR GDRPDAASQT PQTSSDWLEQ VHSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ankib1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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Ankyrin Repeat and IBR Domain Containing 1 (ANKIB1) (AA 2-1089) protein (His tag)
Crystallography grade ANKIB1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ANKIB1 (Ankyrin Repeat and IBR Domain Containing 1 (ANKIB1))
- Alternative Name
- Ankib1 (ANKIB1 Products)
- Synonyms
- ANKIB1 Protein, 2310061P20Rik Protein, 4631416I11Rik Protein, AW494740 Protein, C80642 Protein, mKIAA1386 Protein, ankib1 Protein, si:dkey-231l1.6 Protein, ankyrin repeat and IBR domain containing 1 Protein, ankyrin repeat and IBR domain containing 1b Protein, ankyrin repeat and IBR domain containing 1a Protein, Ankib1 Protein, ANKIB1 Protein, ankib1b Protein, ankib1 Protein, ankib1a Protein
- Background
- Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. {ECO:0000250}.
- Molecular Weight
- 122.7 kDa Including tag.
- UniProt
- Q6ZPS6
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