TPBG Protein (AA 32-355) (His-Avi Tag,Biotin)
-
- Target See all TPBG Proteins
- TPBG (Trophoblast Glycoprotein (TPBG))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-355
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TPBG protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human TPBG/5T4 Protein
- Sequence
- Ser32-Ser355
- Characteristics
- Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA. See testing image for detail.
-
Trophoblast Glycoprotein (TPBG) (AA 32-355) protein (His-Avi Tag)
TPBG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Trophoblast Glycoprotein (TPBG) (AA 35-355) protein (His tag)TPBG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Trophoblast Glycoprotein (TPBG) protein (His tag)TPBG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Trophoblast Glycoprotein (TPBG) (AA 1-420) protein (GST tag)TPBG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Trophoblast Glycoprotein (TPBG) (AA 219-328) protein (GST tag)TPBG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Trophoblast Glycoprotein (TPBG) protein (mFc Tag)TPBG Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Trophoblast Glycoprotein (TPBG) (AA 32-355) protein (FITC,His-Avi Tag)TPBG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Trophoblast Glycoprotein (TPBG) protein (His tag,Biotin)TPBG Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Trophoblast Glycoprotein (TPBG) (AA 32-355) protein (His tag)Crystallography grade TPBG Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Trophoblast Glycoprotein (TPBG) (AA 32-420) protein (rho-1D4 tag)Crystallography grade TPBG Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- TPBG (Trophoblast Glycoprotein (TPBG))
- Alternative Name
- TPBG (TPBG Products)
- Synonyms
- 5T4 Protein, 5T4AG Protein, M6P1 Protein, AW495680 Protein, TPBG Protein, tpbg Protein, trophoblast glycoprotein Protein, trophoblast glycoprotein b Protein, TPBG Protein, Tpbg Protein, tpbgb Protein, tpbg Protein
- Background
- The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
- Molecular Weight
- 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
-
