TRPC5 antibody (AA 827-845) (FITC)
(1 validation)-
- Target See all TRPC5 Antibodies
- TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))
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Binding Specificity
- AA 827-845
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TRPC5 antibody is conjugated to FITC
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Antibody Array (AA)
- Specificity
- Detects ~110 kDa.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)
- Clone
- N67-15 (Formerly S67-15)
- Isotype
- IgG2b
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anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody
TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (HRP)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) HRP
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (Biotin)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) Biotin
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (PE)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) PE
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (APC)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) APC
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (PerCP)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) PerCP
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (Atto 488)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) Atto 488
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (Atto 594)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) Atto 594
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (Atto 390)TRPC5 Reactivity: Human WB, IHC, IP, AA Host: Mouse Monoclonal N67-15 (Formerly S67-15) Atto 390
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 534-603) antibodyTRPC5 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1C8 unconjugated
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- Application Notes
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- WB (1:1000)
- IHC (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2485379 was sufficient for detection of TrpC5 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- by
- Experimentelle Anästhesiologie, Universitätsklinikum Erlangen
- No.
- #103875
- Date
- 04/10/2019
- Antigen
- TRPC5
- Lot Number
- 1007
- Method validated
- Immunofluorescence
- Positive Control
C57Bl6/J Mouse WT Trigeminal Ganglion (TG)
- Negative Control
Mouse TRPC5 knock-out Trigeminal Ganglion
- Notes
ABIN2485379 specifically stains TRPC5 positive neurons in fixed murine trigeminal ganglia.
- Primary Antibody
- ABIN2485379
- Secondary Antibody
- Full Protocol
- Surgical plane of anesthesia with Ketamine and Xylazine.
- Perfuse mouse transcardially with
- 1ml/g BW ice cold PBS
- 1ml/g BW 4% PFA.
- Postfix skull base in 4% PFA ON at 4°C.
- Harvest TGs in PBS.
- Wash TGs 4x with PBS for 30min at RT.
- Incubate TGs with rotator in blocking solution (PBS containing 1% BSA, 0.5% Triton X-100) ON at 4°C.
- Incubate TGs with rotator with primary FITC-conjugated mouse anti-TRPC5 antibody (antibodies-online, ABIN2485379, lot 1007) diluted 1:500 in blocking solution ON at 4°C.
- Wash TGs 4x with PBS for 30min at RT.
- Mount TGs with 1% low melting agarose.
- Whole Organ 2-Photon Imaging with a Zeiss LSM880 NLO Intravital Microscope; Settings: Excitation Wavelenth: 940 nm, Detection: 500-550 nm for wt and TRPC5 ko TGs.
- Experimental Notes
In wt mice antibody concentrations of 1:100, 1:500, 1:1000 and 1:2000 were tested with best results using a concentration of 1:500.
ABIN2485379 is suitable for whole organ IHC. TRPC5 positive Neurons show clear cytoplasmic and membranous staining. No neuronal staining visible. Residues of antibody visible in vasculature due to tissue manipulation during washing.
Validation #103875 (Immunofluorescence)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![A. Representative image of ophthalmic region of trigeminal ganglion of C57Bl6/J mouse stained with ABIN2485379 diluted 1:500. B. Representative image of Z-Stack of ophthalmic region of trigeminal ganglion of TRPC5 ko stained with ABIN2485379 diluted 1:500.]()
A. Representative image of ophthalmic region of trigeminal ganglion of C57Bl6/J mouse stained with ABIN2485379 diluted 1:500. B. Representative image of Z-Stack of ophthalmic region of trigeminal ganglion of TRPC5 ko stained with ABIN2485379 diluted 1:500.
Full Methods -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))
- Alternative Name
- TRPC5 (TRPC5 Products)
- Synonyms
- trpc5 antibody, wu:fi73f08 antibody, TRPC5 antibody, Trpc5 antibody, TRP5 antibody, CCE2 antibody, TRP-5 antibody, Trrp5 antibody, trp antibody, transient receptor potential cation channel, subfamily C, member 5a antibody, transient receptor potential cation channel subfamily C member 5 antibody, transient receptor potential cation channel, subfamily C, member 5 antibody, trpc5a antibody, TRPC5 antibody, Trpc5 antibody
- Background
- Transient receptor potential cation channel, subfamily C, member 5, also known as TRPC5, is a subtype of the TRPC family of mammalian transient receptor potential ion channels. Homo-multimeric TRPC5 and hetero-multimeric TRPC5-TRPC1 channels are activated by extracellular reduced thioredoxin (1). This activation probably plays a role in rheumatoid arthritis. It has also been recently found to be involved in the action on anaesthetics such as chloroform, halothane and propofol (2).
- Gene ID
- 7224
- NCBI Accession
- NP_036603
- UniProt
- Q9UL62
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