C5AR1 antibody (C-Term)
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- Target See all C5AR1 Antibodies
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This C5AR1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- C5AR1/CD88
- Sequence
- RESKSFTRST VDT
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
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anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody
C5AR1 Reactivity: Human, Cow, Rabbit, Ferret WB, FACS, IHC (p), IP Host: Mouse Monoclonal S5-1 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4E2 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 250-350) antibodyC5AR1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-36) antibodyC5AR1 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (FITC)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 FITC
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 175-201) antibodyC5AR1 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB24276 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) antibodyC5AR1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (APC)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 APC
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (PE)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 PE
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 38 kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3 kDa according to NP_001727.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/m
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
- Alternative Name
- C5AR1 (C5AR1 Products)
- Synonyms
- C5A antibody, C5AR antibody, C5R1 antibody, CD88 antibody, C5aR antibody, C5r1 antibody, Cd88 antibody, D7Msu1 antibody, GPR77 antibody, complement C5a receptor 1 antibody, complement component 5a receptor 1 antibody, C5AR1 antibody, C5ar1 antibody, c5ar1 antibody
- Background
- C5AR1, C5A, C5AR, C5A-R, C5R1, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, Complement component 5 receptor 1, Complement component 5a receptor 1, Anaphylatoxin receptor
- Gene ID
- 728, 113959
- NCBI Accession
- NP_001727
- Pathways
- Complement System
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