Ubiquitin B antibody (AA 1-119)
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- Target See all Ubiquitin B (UBB) Antibodies
- Ubiquitin B (UBB)
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Binding Specificity
- AA 1-119
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ubiquitin B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
- Clone
- UBB-1748
- Isotype
- IgG2c kappa
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anti-Ubiquitin B (UBB) antibody
UBB Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 3C12 unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB Reactivity: Human WB, ELISA, IHC, StM, Coat Host: Mouse Monoclonal UBB-2122 unconjugated
anti-Ubiquitin B (UBB) (C-Term) antibodyUBB Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1F5 unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 77-152) antibodyUBB Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 103-228) antibodyUBB Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB Reactivity: Human IHC, StM Host: Rabbit Monoclonal UBB-3143R unconjugated Recombinant Antibody
anti-Ubiquitin B (UBB) (N-Term) antibodyUBB Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin B (UBB) antibodyUBB Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Positive Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.
Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Ubiquitin B (UBB)
- Alternative Name
- UBB (UBB Products)
- Background
- Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.
- Molecular Weight
- 9kDa
- Gene ID
- 7314
- UniProt
- P62979, P62987, P62988
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway
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