UCHL3 antibody (C-Term)
(4 references)-
- Target See all UCHL3 (Uchl3) Antibodies
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
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Binding Specificity
- AA 195-225, C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This UCHL3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Predicted Reactivity
- B, M, Pig, Rat
- Purification
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.
- Clone
- RB4187
- Isotype
- Ig Fraction
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anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibody
Uchl3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4E9 unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 195-225) antibodyUchl3 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibodyUchl3 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibodyUchl3 Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220) antibodyUchl3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220), (C-Term) antibodyUchl3 Reactivity: Human IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibodyUchl3 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (C-Term) antibodyUchl3 Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Monkey, Pig, Hamster IHC, IHC (p), IF, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibodyUchl3 Reactivity: Human, Rat, Mouse IHC Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (N-Term) antibodyUchl3 Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 6 months
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: "Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11314-25, (2010) (PubMed).
: "Role of UCH-L1/ubiquitin in acute testicular ischemia-reperfusion injury." in: Biochemical and biophysical research communications, Vol. 366, Issue 2, pp. 539-44, (2008) (PubMed).
: "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population." in: Journal of human genetics, Vol. 48, Issue 5, pp. 249-70, (2003) (PubMed).
: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, (1989) (PubMed).
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: "Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11314-25, (2010) (PubMed).
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- Target
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
- Alternative Name
- UCHL3 (Uchl3 Products)
- Synonyms
- UCH-L3 antibody, UCH-6 antibody, RGD1561196 antibody, im:6908825 antibody, zgc:109963 antibody, uchl4 antibody, ubiquitin C-terminal hydrolase L3 antibody, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) antibody, ubiquitin C-terminal hydrolase L3 L homeolog antibody, UCHL3 antibody, Uchl3 antibody, uchl3 antibody, uchl3.L antibody
- Background
- Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.
- Molecular Weight
- 26183
- NCBI Accession
- NP_001257881, NP_005993
- UniProt
- P15374
- Pathways
- Feeding Behaviour, Positive Regulation of fat Cell Differentiation
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