ZAP70 antibody (C-Term)
(1 reference)-
- Target See all ZAP70 Antibodies
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
-
Binding Specificity
- C-Term
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This ZAP70 antibody is un-conjugated
-
Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
- Clone
- ZAP-03
- Isotype
- IgG1
-
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
ZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 1F3G9 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 Reactivity: Human IHC, ELISA, FACS, IF, StM, Coat Host: Mouse Monoclonal ZAP70-2035 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibodyZAP70 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibodyZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6D9C2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibodyZAP70 Reactivity: Human IHC, FACS, IF, StM Host: Mouse Monoclonal 2F3-2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibodyZAP70 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibodyZAP70 Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 3D2A4 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 Reactivity: Human IHC, ELISA, StM, Coat Host: Mouse Monoclonal ZAP70-2046 unconjugated
-
- Application Notes
-
Flow cytometry: Intracellular staining, recommended dilution: 2-5 μg/mL, positive control: HPB-ALL human peripheral blood T cell leukemia cell line.
Western blotting: Recommended dilution: 0.5-2 μg/mL. - Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
-
-
: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).
-
: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).
-
- Target
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- Alternative Name
- ZAP-70 (ZAP70 Products)
- Background
- Zeta chain of T cell receptor associated protein k,The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.,ZAP-70, ADMIO2
- Gene ID
- 7535
- UniProt
- P43403
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
-
