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Calretinin antibody

Name: Calretinin antibody
Brand: antibodies-online
SKU: ABIN2855596
Price: 609.18 USD
Availability: InStock
Rating: 5 (1 reviews)

CALB2 Reactivity: Human WB, IHC (p), IF, ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

IHC

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

IHC

7 images

(1 reference)

(1 validation)

Catalog No. ABIN2855596

Datasheet as PDF

Target See all Calretinin (CALB2) Antibodies

Calretinin (CALB2) (Calbindin 2 (CALB2))
Reactivity
82

55

55

8

4

4

4

4

4

3

2

2

2

1

1
Human
Host
62

19

8

1
Rabbit
Compatible Secondaries
Clonality
57

32
Polyclonal
Conjugate
61

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This Calretinin antibody is un-conjugated
Application
70

36

30

16

16

13

13

13

10

7

7

7

6

4

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Characteristics

Rabbit Polyclonal antibody to Calretinin (calbindin 2)
Calretinin antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.

Isotype

IgG
anti-Calbindin 2 (CALB2) (AA 28-248) antibody
CALB2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Calbindin 2 (CALB2) (AA 23-242) antibody
CALB2 Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CALB2-2602 unconjugated

anti-Calbindin 2 (CALB2) (AA 1-271) antibody
CALB2 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Calbindin 2 (CALB2) (AA 172-271) antibody
CALB2 Reactivity: Human WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 7F12G9 unconjugated

anti-Calbindin 2 (CALB2) (AA 172-271) antibody
CALB2 Reactivity: Human, Monkey WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 7H1G3 unconjugated

anti-Calbindin 2 (CALB2) (AA 1-271) antibody
CALB2 Reactivity: Human WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 1F5H1 unconjugated

anti-Calbindin 2 (CALB2) antibody
CALB2 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Calbindin 2 (CALB2) (AA 211-271) antibody
CALB2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Calbindin 2 (CALB2) antibody
CALB2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Calbindin 2 (CALB2) antibody
Microarray verified CALB2 Reactivity: Human, Mouse IHC Host: Mouse Monoclonal MAB539585 unconjugated

Application Notes

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: A431 , Mouse brain , human CALB2-transfected 293T cells , rat brain
Validation: Overexpression

Restrictions

For Research Use only
Validation #103867 (Immunohistochemistry)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#103867

Date

05/29/2019

Antigen

Calretinin

Lot Number

39876

Method validated

Immunohistochemistry

Positive Control

Adult (24 months) mouse and dog brain

Negative Control

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Notes

ABIN2855596 works in IHC-P with microwave antigen retrieval pretreatment at an optimal concentration of 1:200.

Validation Images

Staining of dog and mouse brain sections using ABIN2855596. A-C: Specific staining of calretinin-immunoreactive cortical neurons in dog brain sections at 20x, 40x, and 63x magnification. D. Staining of calretinin-immunoreactive cortical neurons in mouse brain sections at 40x magnification.
Full Methods
Primary Antibody

ABIN2855596

Secondary Antibody

goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)

Full Protocol

Obtain dog samples after autopsy and immersion-fix in buffered formaldehyde for 16h.
Perfuse mice with paraformaldehyde 4% in 0.1M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2h at RT.
Wash, dehydrate, and embed samples in paraffin wax.
Cut with a microtome into 6µm sections and mount on glass slides.
After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer (pH 6.0).
Let sections cool for 10min.
Wash sections under running water for 5-10min.
Wash sections 2x 5min with distilled water.
Wash sections 5min with 0.01M PBS.
Incubate sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) for 1h at RT to block non-specific binding sites.
Incubate sections with primary rabbit anti-Calretinin antibody (antibodies-online, ABIN2855596, lot 39876) diluted 1:200 in PBS-BSA-PLL at 4°C.
Wash sections 3x 5min with 0.01M PBS.
Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1M PBS for 1h at RT.
Wash sections 3x 5min with 0.01M PBS.
Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x, 40x, and 63x. Parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.

Experimental Notes

For the incubation with the primary antibody ABIN2669973 1:100, 1:500, and 1:1000 dilutions were also tested.
Format

Liquid

Concentration

0.75 mg/mL

Buffer

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Bradshaw, Trossbach, Köber, Walter, Prikulis, Weggen, Korth: "Disrupted in Schizophrenia 1 regulates the processing of reelin in the perinatal cortex." in: Schizophrenia research, Vol. 215, pp. 506-513, (2021) (PubMed).
Target

Calretinin (CALB2) (Calbindin 2 (CALB2))

Alternative Name

calbindin 2 (CALB2 Products)

Synonyms

CAB29 antibody, CAL2 antibody, CR antibody, calb2l antibody, wu:fq18e08 antibody, zgc:73115 antibody, calb2 antibody, wu:fq17g09 antibody, zgc:73099 antibody, calbindin 2 antibody, calbindin 2a antibody, calbindin 2b antibody, CALB2 antibody, Calb2 antibody, calb2a antibody, calb2b antibody

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described.

Molecular Weight

32 kDa

Gene ID

794

UniProt

P22676