GRB2 antibody
(1 validation)-
- Target See all GRB2 Antibodies
- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
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Reactivity
- Human, Mouse, Rat, Dog, Chicken
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GRB2 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Rat
- Predicted Reactivity
- Mouse,Dog,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human GRB2
- Isotype
- IgG
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anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody
Verified GRB2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC Host: Goat Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibodyKO Validated GRB2 Reactivity: Human WB, IHC (p), IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 Reactivity: Human WB, IHC, IP, IF Host: Rabbit Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 89-118) antibodyGRB2 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 187-216) antibodyGRB2 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB11305-11306 unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 89-118) antibodyGRB2 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB15648 unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (Tyr209) antibodyGRB2 Reactivity: Human WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 156-217) antibodyGRB2 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 Reactivity: Human, Mouse, Rat, Cow, Hamster WB, IHC, IHC (p) Host: Mouse Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (N-Term) antibodyGRB2 Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Horse, Rabbit, Guinea Pig WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB(1:100-500)
Optimal working dilution should be determined by the investigator. - Restrictions
- For Research Use only
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- by
- Alamo Laboratories Inc
- No.
- #029728
- Date
- 06/05/2014
- Antigen
- Lot Number
- 121228
- Method validated
- Western Blotting
- Positive Control
- NIH/3T3 cells
- Negative Control
- HeLa cells - very low expression
- Notes
- A strong band was seen at the correct molecular weight in the positive control, and no band was present in the negative control. Additionally, no non-specific bands were observed.
- Primary Antibody
- Antigen: Growth Factor Receptor-Bound Protein 2 (GRB2) (1:200 dilution)
- Catalog number: ABIN686932
- Lot number: 121228
- Secondary Antibody
- Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
- Lot number: L170-6515
- Full Protocol
- Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
- 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
- The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
- Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
- The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
- The membrane was rinsed with TBST once.
- The membrane was immersed with the protein side up in the primary antibody solution (anti-GRB2; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
- The membrane was rinsed in TBST three times for 5 min each.
- The membrane was incubated in the HRP-conjugated secondary antibody solution (goat-anti rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
- The membrane was rinsed three times with TBST for 5 min each.
- The membrane was rinsed in TBS twice for 30 s each.
- Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
- The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
- The membrane was washed in TBST 2 times for 10 min each.
- Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (goat-anti rabbit IgG-HRP; 1:20,000).
- Experimental Notes
- Nothing to note.
Validation #029728 (Western Blotting)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Figure 1: Western blot of lysates from NIH/3T3 cells (Lane 1, positive control) and HeLa cells (Lane 2, negative control) probed with anti-GRB2 (upper panel) or with anti- Actin for loading control (lower panel).]()
Figure 1: Western blot of lysates from NIH/3T3 cells (Lane 1, positive control) and HeLa cells (Lane 2, negative control) probed with anti-GRB2 (upper panel) or with anti- Actin for loading control (lower panel).
Full Methods -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
- Alternative Name
- GRB2/ASH (GRB2 Products)
- Synonyms
- GRB2 antibody, grb2 antibody, AA408164 antibody, Ash antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, wu:fb18e12 antibody, wu:fb34e06 antibody, zgc:73077 antibody, growth factor receptor bound protein 2 antibody, growth factor receptor-bound protein 2 antibody, growth factor receptor-bound protein 2b antibody, GRB2 antibody, grb2 antibody, Grb2 antibody, grb2b antibody
- Background
- Optional[synonyms]: GRB 2, GRB2 adapter protein, Grb3 3, Growth Factor Receptor Bound Protein 2, Growth factor receptor bound protein 3, EGFRBP GRB2, Growth factor receptor-bound protein 2, Abundant SRC homology, Adapter protein GRB2, ASH, Ash protein, EGFRBP GRB2, Epidermal growth factor receptor binding protein, Epidermal growth factor receptor binding protein GRB2, GRB 2, GRB2 adapter protein, Grb3 3, Growth Factor Receptor Bound Protein 2, Growth factor receptor bound protein 3, HT027, MST084, MSTP084, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, Protein ASH, SEM5, SH2/SH3 adapter GRB2.
- Molecular Weight
- 25kDa
- Gene ID
- 2885
- UniProt
- P62993
- Pathways
- RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
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