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SPOP-B antibody (Internal Region)

Name: SPOP-B antibody (Internal Region)
SKU: ABIN6265263
Price: 390.77 USD
Availability: InStock

SPOP-B Reactivity: Human, Mouse, Rat WB, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts of HeLa cells, using SPOP antibody.

ABIN6272950 at 1/100 staining Human breast cancer tissue by IHC-P.

IHC

Western blot analysis of extracts from Hybridoma cells, using SPOP Antibody.

3 images

Catalog No. ABIN6265263

Datasheet as PDF

Target See all SPOP-B Antibodies

SPOP-B (Speckle-Type POZ Protein (SPOP-B))
Binding Specificity
15

8

7

6

5

4

2

2

2

1

1

1

1

1

1
Internal Region
Reactivity
57

17

12

5

3

2

2

2

2

1

1

1

1

1
Human, Mouse, Rat
Host
56

4
Rabbit
Compatible Secondaries
Clonality
56

4
Polyclonal
Conjugate
25

5

5

4

2

2

2

2

2

2

2

2

1

1

1

1

1
This SPOP-B antibody is un-conjugated
Application
48

24

13

13

4

3

3

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

SPOP Antibody detects endogenous levels of total SPOP.

Predicted Reactivity

Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen

A synthesized peptide derived from human SPOP, corresponding to a region within the internal amino acids.

Isotype

IgG
anti-Speckle-Type POZ Protein (SPOP-B) (Center) antibody
SPOP-B Reactivity: Human, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 1-374) antibody
SPOP-B Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 301-374) antibody
SPOP-B Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3E2 unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (C-Term) antibody
SPOP-B Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 48-374) antibody
SPOP-B Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 74-107), (N-Term) antibody
SPOP-B Reactivity: Mouse, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal RB49885 unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 1-374) antibody
SPOP-B Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (C-Term) antibody
SPOP-B Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit, Chicken, Xenopus laevis, Hamster, Monkey, Pig WB Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 1-193) antibody
SPOP-B Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Speckle-Type POZ Protein (SPOP-B) (AA 175-374) antibody
SPOP-B Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Target

SPOP-B (Speckle-Type POZ Protein (SPOP-B))

Alternative Name

SPOP (SPOP-B Products)

Synonyms

BTBD32 antibody, TEF2 antibody, AI315626 antibody, Pcif1 antibody, zgc:64140 antibody, spop antibody, tef2 antibody, speckle type BTB/POZ protein antibody, speckle-type POZ protein antibody, speckle type POZ protein S homeolog antibody, SPOP antibody, Spop antibody, spop antibody, spop.S antibody

Background

Description: Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1, this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.
Gene: SPOP

Molecular Weight

42 kDa

Gene ID

8405

UniProt

O43791