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ADO antibody

ADO Reactivity: Human, Mouse, Rat WB, FACS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5647727
  • Target See all ADO Antibodies
    ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
    Reactivity
    • 34
    • 20
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 34
    Rabbit
    Clonality
    • 34
    Polyclonal
    Conjugate
    • 12
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADO antibody is un-conjugated
    Application
    • 19
    • 18
    • 15
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Purification
    Antigen affinity purified
    Immunogen
    Amino acids E49-E261 from the human protein were used as the immunogen for the ADO antibody.
    Isotype
    IgG
  • Application Notes
    Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the ADO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
    Alternative Name
    ADO / 2-Aminoethanethiol dioxygenase (ADO Products)
    Synonyms
    C10orf22 antibody, Gm237 antibody, RGD1308233 antibody, wu:fc32g01 antibody, wu:fc52c12 antibody, zgc:101580 antibody, 2-aminoethanethiol dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase antibody, 2-aminoethanethiol (cysteamine) dioxygenase a antibody, ADO antibody, Ado antibody, LOC100351598 antibody, adoa antibody
    Background
    Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
    UniProt
    Q96SZ5
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