RUNX3 antibody (AA 31-130)
(1 validation)-
- Target See all RUNX3 Antibodies
- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
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Binding Specificity
- AA 31-130
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RUNX3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Runx3
- Isotype
- IgG
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anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 294-429) antibody
RUNX3 Reactivity: Human WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 7E2B8 unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 Reactivity: Human WB, IF, IHC (p) Host: Mouse Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 128-270) antibodyRUNX3 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-252) antibodyRUNX3 Reactivity: Human, Mouse WB, ELISA, IHC, FACS, IF/ICC Host: Mouse Monoclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 Reactivity: Human WB, IF, IHC (p) Host: Mouse Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 168-197) antibodyRUNX3 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-415) antibodyRUNX3 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (Internal Region) antibodyRUNX3 Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 194-279) antibodyRUNX3 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 294-429) antibodyRUNX3 Reactivity: Human WB, ELISA, IHC, FACS, ICC, Neut Host: Mouse Monoclonal 8C9B6 unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- by
- CaresBio Laboratory
- No.
- #029621
- Date
- 03/08/2014
- Antigen
- Lot Number
- 131016
- Method validated
- Immunofluorescence
- Positive Control
- A431 cells
- Negative Control
- HEK293 cells
- Notes
- Strong signal was detected in the positive control, and no signal was detected in the negative control.
- Primary Antibody
- Antigen: Runt-Related Transcription Factor 3 (RUNX3)
- Catalog number: ABIN1714069
- Lot number: 131016
- Secondary Antibody
- Antibody: Donkey Anti-Rabbit secondary antibody IgG (H+L) AlexaFluor 488
- Full Protocol
- Antibody staining:
- A431 and Hek-293 cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
- Fixed cells were rinsed three times in PBS for 5 min each at RT.
- Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
- Cells were incubated with primary antibody diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
- Stained cells were imaged with an Olympus IX73 microscope.
- Isotype Control Staining:
- All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
- Stained cells were imaged with an Olympus IX73 microscope.
- Secondary Antibody Only Control Staining:
- All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with donkey anti rabbit Alexa488 conjugated secondary antibody for 60 min in dark at RT.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
- Stained cells were imaged with an Olympus IX73 microscope.
- Experimental Notes
- No discrepancies reported.
- Runx3 antibody is recommended for IF on A431 cells according to this protocol.
Validation #029621 (Immunofluorescence)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Figure 1: A431 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).]()
Figure 1: A431 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).
![Figure 2: HEK293 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).]()
Figure 2: HEK293 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).
![Figure 3: A431 cells stained with isotype control (green) and counterstained with DAPI (blue).]()
Figure 3: A431 cells stained with isotype control (green) and counterstained with DAPI (blue).
![Figure 4: A431 cells stained with secondary antibody only (green) and counterstained with DAPI (blue).]()
Figure 4: A431 cells stained with secondary antibody only (green) and counterstained with DAPI (blue).
Full Methods -
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
- Alternative Name
- Runx3 (RUNX3 Products)
- Synonyms
- runxb antibody, RUNX3 antibody, CBFA3 antibody, frrunx3 antibody, runx3 antibody, aml2 antibody, cbfa3 antibody, pebp2ac antibody, AML2 antibody, Cbfa3 antibody, Pebp2a3 antibody, PEBP2aC antibody, runt-related transcription factor 3 antibody, runt related transcription factor 3 antibody, runt related transcription factor 3 L homeolog antibody, runx3 antibody, RUNX3 antibody, runx3.L antibody, Runx3 antibody
- Background
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Synonyms: RUNX 3, RUNX-3, Runt-related transcription factor 3, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, OncogeneAML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.
Background: RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.
- Gene ID
- 864
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