NUMB antibody (C-Term)
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- Target See all NUMB Antibodies
- NUMB (Numb Homolog (NUMB))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NUMB antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- NUMB
- Sequence
- PFSSDLQKTF EIEL
- Specificity
- This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet b
- Cross-Reactivity
- Cow, Dog, Human, Mouse
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1.
- Isotype
- IgG
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anti-Numb Homolog (NUMB) (AA 521-640) antibody
NUMB Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4A7-A6 unconjugated
anti-Numb Homolog (NUMB) (AA 1-50) antibodyNUMB Reactivity: Human, Mouse, Rat, Cow, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (pSer276) antibodyNUMB Reactivity: Mouse WB, ELISA, IF (cc), ICC, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (C-Term) antibodyNUMB Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-30), (N-Term) antibodyNUMB Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB14789 unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-214) antibodyNUMB Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 600-653) antibodyNUMB Reactivity: Human, Mouse, Rat, Chicken WB Host: Rabbit Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 376-482) antibodyNUMB Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 6-8 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cell junctions/plasma membrane and cytoplasm of A431 and NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- NUMB (Numb Homolog (NUMB))
- Alternative Name
- NUMB (NUMB Products)
- Synonyms
- C14orf41 antibody, S171 antibody, c14_5527 antibody, Nb antibody, NUMB, endocytic adaptor protein antibody, numb homolog (Drosophila) antibody, NUMB antibody, Numb antibody, numb antibody
- Background
- NUMB, numb homolog (Drosophila), S171, numb homolog
- Gene ID
- 8650, 18222
- NCBI Accession
- NP_001005743, NP_003735, NP_001005745
- Pathways
- Cell-Cell Junction Organization
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