PDE5A antibody (C-Term)
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- Target See all PDE5A Antibodies
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PDE5A antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- PDE5A
- Sequence
- LINGESGQAK RN
- Specificity
- This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
- Cross-Reactivity
- Cow, Dog, Human, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
- Isotype
- IgG
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anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 29-138) antibody
PDE5A Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 9H5 unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) antibodyPDE5A Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) antibodyPDE5A Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 303-442) antibodyPDE5A Reactivity: Human IF, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) WB, IHC Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 101-200) antibodyPDE5A Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A Reactivity: Human, Rat, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 1-150) antibodyPDE5A Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) antibodyPDE5A WB, IHC, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
- Alternative Name
- PDE5A (PDE5A Products)
- Background
- PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
- Gene ID
- 8654
- NCBI Accession
- NP_001074, NP_246273, NP_236914
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
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