TNFSF13 Protein (Trimer) (His-DYKDDDDK Tag)
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- Target See all TNFSF13 Proteins
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF13 protein is labelled with His-DYKDDDDK Tag.
- Purpose
- Human APRIL/TNFSF13 Trimer Protein
- Sequence
- Lys112-Leu250
- Characteristics
- Recombinant Human APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Lys112-Leu250.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human APRIL Trimer, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 96-241) protein (Fc Tag)
TNFSF13 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Trimer) protein (His-Avi-DYKDDDDK Tag)TNFSF13 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 1-247) protein (GST tag)TNFSF13 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 111-250) (Active) protein (His tag,DYKDDDDK Tag)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) (Active) protein (Fc Tag,AVI tag,Biotin)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Trimer) protein (His-Avi-DYKDDDDK Tag,Biotin)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- Alternative Name
- APRIL (TNFSF13 Products)
- Synonyms
- APRIL Protein, CD256 Protein, TALL-2 Protein, TALL2 Protein, TRDL-1 Protein, ZTNF2 Protein, 2310026N09Rik Protein, April Protein, Tall2 Protein, Trdl1 Protein, SLAN Protein, ANP32B Protein, april Protein, tnf13 Protein, TNLG7B Protein, TNF superfamily member 13 Protein, tumor necrosis factor (ligand) superfamily, member 13 Protein, acidic leucine-rich nuclear phosphoprotein 32 family member B Protein, TNFSF13 Protein, Tnfsf13 Protein, LOC494442 Protein, tnfsf13 Protein
- Background
- The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
- Molecular Weight
- 49.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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