TRAIL Protein (AA 114-281)
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- Target See all TRAIL (TNFSF10) Proteins
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 114-281
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Human TRAIL/TNFRSF10 Protein
- Sequence
- Val114-Gly281
- Characteristics
- Recombinant Human TRAIL/TNFRSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human TRAIL at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA. The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (Trimer) protein (His-DYKDDDDK Tag)
TNFSF10 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-281) protein (GST tag)TNFSF10 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 114-281) protein (His-DYKDDDDK-Strep II Tag)TNFSF10 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 118-291) protein (His tag)TNFSF10 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) protein (His tag)TNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 39-281) protein (His tag)Crystallography grade TNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 39-291) protein (His tag)Crystallography grade TNFSF10 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-281) protein (Strep Tag)Crystallography grade TNFSF10 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-291) protein (Strep Tag)Crystallography grade TNFSF10 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in 50 mM Tris, 300 mM NaCl ( pH 7.2).
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Alternative Name
- TRAIL (TNFSF10 Products)
- Synonyms
- APO2L Protein, Apo-2L Protein, CD253 Protein, TL2 Protein, TRAIL Protein, A330042I21Rik Protein, AI448571 Protein, APO-2L Protein, Ly81 Protein, Trail Protein, tl2 Protein, apo2l Protein, cd253 Protein, trail Protein, apo-2l Protein, trail1 Protein, xtrail1 Protein, tnfsf10l2 Protein, zgc:92320 Protein, TNLG6A Protein, TNF superfamily member 10 Protein, tumor necrosis factor (ligand) superfamily, member 10 Protein, tumor necrosis factor superfamily member 10 Protein, TNF superfamily member 10 S homeolog Protein, TNFSF10 Protein, Tnfsf10 Protein, tnfsf10.S Protein, tnfsf10 Protein
- Background
- Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.
- Molecular Weight
- 19.6 kDa same as Tris-Bis PAGE result.
- NCBI Accession
- NP_003801
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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