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SPHK1 antibody (AA 286-315)

SPHK1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal RB03621 unconjugated
Catalog No. ABIN391346
  • Target See all SPHK1 Antibodies
    SPHK1 (Sphingosine Kinase 1 (SPHK1))
    Binding Specificity
    • 15
    • 14
    • 9
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 286-315
    Reactivity
    • 103
    • 42
    • 37
    • 11
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 95
    • 8
    • 1
    Rabbit
    Clonality
    • 91
    • 13
    Polyclonal
    Conjugate
    • 54
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This SPHK1 antibody is un-conjugated
    Application
    • 75
    • 46
    • 25
    • 18
    • 13
    • 13
    • 13
    • 10
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    This antibody is purified through a protein A column, followed by peptide affinity purification.
    Immunogen
    This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
    Clone
    RB03621
    Isotype
    Ig Fraction
  • Application Notes
    WB: 1:2000. WB: 1:1000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:25
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
    Expiry Date
    6 months
  • Furuya, Shimizu, Tamashiro, Iino, Bielawski, Chan, Pagano, Kawamori: "Sphingosine kinase 1 expression enhances colon tumor growth." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 120, (2018) (PubMed).

    Song, Zhang, Chen, Xia, Qin, Waeber, Sheng: "Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain." in: Cell death & disease, Vol. 8, Issue 7, pp. e2912, (2018) (PubMed).

    Zhu, You, Tan, Li, Qiu, Li, Huang, Zheng, Ren: "Overexpression of sphingosine kinase 1 is predictive of poor prognosis in human breast cancer." in: Oncology letters, Vol. 14, Issue 1, pp. 63-72, (2017) (PubMed).

    Do, Kim, Kim, Lee, Do, Kim, Chae, Sohn: "Predictive Value of Sphingosine Kinase 1 Expression in Papillary Thyroid Carcinoma." in: Anticancer research, Vol. 37, Issue 10, pp. 5399-5405, (2017) (PubMed).

    Gairhe, Joshi, Bastola, McLendon, Oka, Fagan, McMurtry: "Sphingosine-1-phosphate is involved in the occlusive arteriopathy of pulmonary arterial hypertension." in: Pulmonary circulation, Vol. 6, Issue 3, pp. 369-80, (2016) (PubMed).

    Dick, Hengst, Fox, Colledge, Kale, Sung, Sharma, Amin, Loughran, Kester, Wang, Yun: "The apoptotic mechanism of action of the sphingosine kinase 1 selective inhibitor SKI-178 in human acute myeloid leukemia cell lines." in: The Journal of pharmacology and experimental therapeutics, Vol. 352, Issue 3, pp. 494-508, (2015) (PubMed).

    Zheng, Wei, Wang, Xu, Xiao, Liu, Jia, Cheng: "Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia." in: Experimental neurology, (2015) (PubMed).

    Matula, Collie-Duguid, Murray, Parikh, Grabsch, Tan, Lalwani, Garau, Ong, Bain, Smith, Urquhart, Bielawski, Finnegan, Petty: "Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer." in: BMC cancer, Vol. 15, pp. 762, (2015) (PubMed).

    Xiong, Wang, Guan, Wu, Xu, Wang, Rong, Huang, Huang, Liao, Fu, Yuan: "SphK1 confers resistance to apoptosis in gastric cancer cells by downregulating Bim via stimulating Akt/FoxO3a signaling." in: Oncology reports, Vol. 32, Issue 4, pp. 1369-73, (2014) (PubMed).

    Altay, Suzuki, Hasegawa, Caner, Krafft, Fujii, Tang, Zhang: "Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2513-6, (2012) (PubMed).

    Fischer, Alliod, Martinier, Newcombe, Brana, Pouly: "Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions." in: PLoS ONE, Vol. 6, Issue 8, pp. e23905, (2011) (PubMed).

    Song, Xiong, Li, Liao, Wang, Wu, Li: "Sphingosine kinase-1 enhances resistance to apoptosis through activation of PI3K/Akt/NF-?B pathway in human non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 7, pp. 1839-49, (2011) (PubMed).

    Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury." in: Anesthesiology, Vol. 114, Issue 2, pp. 363-73, (2011) (PubMed).

    Kim, Park, Kim, DAgati, Lee: "Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction." in: American journal of physiology. Renal physiology, Vol. 300, Issue 1, pp. F167-76, (2011) (PubMed).

    Nayak, Huo, Kwang, Pushparaj, Kumar, Ling, Dheen: "Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia." in: Neuroscience, Vol. 166, Issue 1, pp. 132-44, (2010) (PubMed).

    Liu, Zheng, Zhang, Wu, Xiong, Li, Song: "Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy." in: BMC cancer, Vol. 10, pp. 495, (2010) (PubMed).

    Crousillac, Colonna, McMains, Dewey, Gleason: "Sphingosine-1-phosphate elicits receptor-dependent calcium signaling in retinal amacrine cells." in: Journal of neurophysiology, Vol. 102, Issue 6, pp. 3295-309, (2009) (PubMed).

    Wadgaonkar, Patel, Grinkina, Romano, Liu, Zhao, Sammani, Garcia, Natarajan: "Differential regulation of sphingosine kinases 1 and 2 in lung injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L603-13, (2009) (PubMed).

    Li, Yu, Xia, Zhang, Weng, Zheng, Kong, Hu, Zeng, Zeng, Li, Li, Song: "Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 4, pp. 1393-9, (2009) (PubMed).

    Li, Guan, Gong, Song, Zhang, Wu, Yuan, Zheng, Huang, Li: "Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 21, pp. 6996-7003, (2008) (PubMed).

  • Target
    SPHK1 (Sphingosine Kinase 1 (SPHK1))
    Alternative Name
    SPHK1 (SPHK1 Products)
    Synonyms
    CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody
    Background
    Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
    Gene ID
    8877
    NCBI Accession
    NP_001136073, NP_001136074, NP_068807, NP_892010
    UniProt
    Q9NYA1
    Pathways
    VEGF Signaling
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