USP13 antibody (C-Term)
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- Target See all USP13 Antibodies
- USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This USP13 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Specificity
- USP13 Antibody detects endogenous levels of total USP13.
- Predicted Reactivity
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human USP13, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
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anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) antibody
USP13 Reactivity: Human WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) antibodyUSP13 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) (AA 389-419) antibodyUSP13 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB4304 unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) (N-Term) antibodyUSP13 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) (AA 500-550) antibodyUSP13 Reactivity: Human, Mouse, Dog, Horse, Cow, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) antibodyUSP13 Reactivity: Human, Dog, Monkey WB, IHC, IP Host: Mouse Monoclonal 4G11 unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) antibodyUSP13 Reactivity: Rat IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) (AA 387-420) antibodyUSP13 Reactivity: Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal RB52034 unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) antibodyUSP13 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13) (AA 1-863) antibodyUSP13 Reactivity: Human WB Host: Mouse Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:1000, IHC: 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))
- Alternative Name
- USP13 (USP13 Products)
- Synonyms
- ISOT3 antibody, IsoT-3 antibody, 2700071E21Rik antibody, AI848077 antibody, usp13 antibody, MGC163140 antibody, ubiquitin specific peptidase 13 antibody, ubiquitin specific peptidase 13 (isopeptidase T-3) antibody, ubiquitin carboxyl-terminal hydrolase 13 antibody, universal stress protein antibody, USP13 antibody, Usp13 antibody, usp13 antibody, MGYG_01656 antibody, RR_RS02895 antibody
- Background
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Description: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.
Gene: USP13
- Molecular Weight
- 97 kDa
- Gene ID
- 8975
- UniProt
- Q92995
- Pathways
- SARS-CoV-2 Protein Interactome
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