SART1 antibody (Internal Region)
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- Target See all SART1 Antibodies
- SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SART1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Characteristics
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Rabbit Polyclonal antibody to SART1 (squamous cell carcinoma antigen recognized by T cells)
SART1 antibody [N2C1], Internal - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human SART1. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (AA 1-255) antibody
SART1 Reactivity: Human WB, IP, IHC Host: Rabbit Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (AA 1-800) antibodySART1 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (C-Term) antibodySART1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) antibodySART1 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (N-Term) antibodyVerified SART1 Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) (C-Term) antibodySART1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) antibodySART1 Reactivity: Human, Mouse, Rat WB, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*
- Comment
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Conjugation Note: MH
Positive Control: Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , NIH-3T3 , JC , BCL-1 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.76 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- SART1 (Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1))
- Alternative Name
- SART1 (SART1 Products)
- Synonyms
- Dmel\\CG6686 antibody, SART1 antibody, MGC53679 antibody, MGC132129 antibody, zgc:91927 antibody, Ara1 antibody, HOMS1 antibody, SART1259 antibody, SNRNP110 antibody, Snu66 antibody, U5-110K antibody, CG6686 gene product from transcript CG6686-RB antibody, SART1, U4/U6.U5 tri-snRNP-associated protein 1 S homeolog antibody, SART1, U4/U6.U5 tri-snRNP-associated protein 1 antibody, squamous-cell carcinoma T-cell-recognized antigen antibody, squamous cell carcinoma antigen recognized by T cells 1 antibody, CG6686 antibody, sart1.S antibody, sart1 antibody, SART1 antibody, Sart1 antibody
- Background
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This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation, its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
Cellular Localization: Nucleus - Molecular Weight
- 90 kDa
- Gene ID
- 9092
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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