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CD1b antibody

Name: CD1b antibody
Brand: antibodies-online
SKU: ABIN1981866
Price: 333.96 USD
Availability: InStock

CD1B Reactivity: Human FACS, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal SN13 unconjugated

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) purified antibody (concentration in sample 9 μg/mL, GAM PE).

FACS

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) purified antibody (concentration in sample 9 μg/mL, GAM PE) and anti-human CD14 (MEM-15) Pacific Blue antibody (4 μL reagent per milion cells in 100 μL of cell suspension).

FACS

Separation of cells stained using anti-human CD1b (SN13) purified antibody (concentration in sample 9 μg/mL, GAM PE, red-filled) from cells unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

FACS

4 images

(3 references)

Catalog No. ABIN1981866

Datasheet as PDF

Target See all CD1b (CD1B) Antibodies

CD1b (CD1B)
Reactivity
59

2

2

2

2

2

2

2
Human
Host
34

26
Mouse
Compatible Secondaries
Clonality
35

25
Monoclonal
Conjugate
29

5

4

4

4

2

1

1

1

1

1

1

1

1

1

1

1

1
This CD1b antibody is un-conjugated
Application
27

24

21

14

7

5

3

2

2

2

2
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

A cell membrane antigen preparation that was isolated from normal human thymocytes

Clone

SN13

Isotype

IgG1 kappa
anti-CD1b (CD1B) (AA 18-303) antibody
CD1B Reactivity: Human FACS, WB, ELISA, IHC, ICC, Neut Host: Mouse Monoclonal 5G3D4 unconjugated

anti-CD1b (CD1B) (AA 18-303) antibody
CD1B Reactivity: Human FACS, WB, ELISA, IHC, ICC, Neut Host: Mouse Monoclonal 1D6B7 unconjugated

anti-CD1b (CD1B) antibody (APC)
CD1B Reactivity: Human FACS Host: Mouse Monoclonal SN13 APC

anti-CD1b (CD1B) antibody (FITC)
CD1B Reactivity: Human FACS Host: Mouse Monoclonal SN13 FITC

anti-CD1b (CD1B) (AA 1-333) antibody
CD1B Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-CD1b (CD1B) antibody
CD1B Reactivity: Human FACS, ELISA, IF, Coat Host: Mouse Monoclonal RIV12 unconjugated

anti-CD1b (CD1B) (AA 18-303) antibody
CD1B Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-CD1b (CD1B) (AA 57-85), (N-Term) antibody
CD1B Reactivity: Human WB Host: Rabbit Polyclonal RB36689 unconjugated

anti-CD1b (CD1B) antibody (PE)
CD1B Reactivity: Human FACS Host: Mouse Monoclonal SN13 PE

anti-CD1b (CD1B) antibody
CD1B Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze. Do not use after expiration date stamped on vial label.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Giuliani, Prete, Graziani, Aquino, Balduzzi, Sugita, Brenner, Iona, Fattorini, Orefici, Porcelli, Bonmassar: "Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells." in: Infection and immunity, Vol. 69, Issue 12, pp. 7461-70, (2001) (PubMed).

Hayes, Knight: "Group 1 CD1 genes in rabbit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 403-10, (2001) (PubMed).

Tentori, Graziani, Porcelli, Sugita, Brenner, Madaio, Bonmassar, Giuliani, Aquino: "Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes." in: Antimicrobial agents and chemotherapy, Vol. 42, Issue 3, pp. 550-4, (1998) (PubMed).
Target

CD1b (CD1B)

Alternative Name

CD1b (CD1B Products)

Synonyms

CD1 antibody, CD1A antibody, R1 antibody, CD1B3 antibody, CD1B antibody, CD1-b antibody, CD1b molecule antibody, T-cell surface glycoprotein CD1b-3 antibody, CD1b1 molecule antibody, T-cell surface glycoprotein CD1b1 antibody, CD1B antibody, LOC443423 antibody, CD1B1 antibody, LOC100720602 antibody

Background

CD1b molecule,CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.,R1

Gene ID

910

UniProt

P29016