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KLF4 antibody (C-Term)

Name: KLF4 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2855075
Price: 609.18 USD
Availability: InStock

KLF4 Reactivity: Human, Mouse WB, FACS, IP Host: Rabbit Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE antibody diluted at 1:3000

IP Image Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of KLF4 antibody, Western blot analysis was performed using KLF4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

WB Image Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE antibody diluted at 1:5000

FACS

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000

WB Image HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody , diluted at 1:2000.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:3000

DDX3 suppresses stemness gene signature.

8 images

(1 reference)

Catalog No. ABIN2855075

Datasheet as PDF

Target See all KLF4 Antibodies

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
Binding Specificity
13

13

11

7

6

6

6

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
109

30

22

8

7

6

6

6

5

4

3

3

2

2

1

1

1

1
Human, Mouse
Host
90

23

1
Rabbit
Compatible Secondaries
Clonality
82

32
Polyclonal
Conjugate
73

8

7

7

7

5

1

1

1

1

1

1

1
This KLF4 antibody is un-conjugated
Application
84

59

49

25

23

20

14

12

6

6

2

2

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Cross-Reactivity

Rat (Rattus), Cat (Feline), Pig (Porcine), Sheep (Ovine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details)

Rat (100 %), Cat (100 %), Pig (100 %), Sheep (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics

Rabbit Polyclonal antibody to KLF4 (Kruppel-like factor 4 (gut))
KLF4 antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary.

Isotype

IgG
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB14263 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivity: Human WB, ELISA, IF, IHC, IP Host: Rabbit Monoclonal 3A1 unconjugated Recombinant Antibody

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) antibody
KLF4 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal X1 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (N-Term) antibody
KLF4 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody
KLF4 Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio) WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibody
KLF4 Reactivity: Human, Mouse WB, ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17718 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibody
KLF4 Reactivity: Human WB, IF Host: Rabbit Polyclonal RB18934 unconjugated

anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibody
KLF4 Reactivity: Human, Mouse WB, IF, FACS Host: Mouse Monoclonal 56CT5-1-6 unconjugated

Application Notes

Suggested dilution Reference Flow cytometry/FACS 1:50-1:200* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACS1:50-1:200* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment

Positive Control: A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , human ESC , mouse ESC , HCT116 , HCT116 nuclear extract , *MDM-MB-231

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Nishimura, Okamoto, Shibue, Mizuta, Shibayama, Yoshino, Murakami, Yamaguchi, Tanaka, Toida, Igarashi: "KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation." in: PLoS ONE, Vol. 15, Issue 2, pp. e0229744, (2020) (PubMed).
Target

KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name

KLF4 (KLF4 Products)

Synonyms

EZF antibody, GKLF antibody, Gklf antibody, Zie antibody, KLF4 antibody, biklf antibody, biklf-A antibody, Kruppel like factor 4 antibody, Kruppel-like factor 4 (gut) antibody, Kruppel-like factor 4 antibody, Kruppel-like factor 4 (gut) L homeolog antibody, KLF4 antibody, Klf4 antibody, klf4 antibody, klf4.L antibody

Background

Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Cellular Localization: Nucleus

Molecular Weight

55 kDa

Gene ID

9314

Pathways

Peptide Hormone Metabolism, Stem Cell Maintenance