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Osteoactivin antibody (AA 81-180)

Name: Osteoactivin antibody (AA 81-180)
SKU: ABIN749093
Price: 384.62 USD
Availability: InStock
Rating: 5 (1 reviews)

GPNMB Reactivity: Human, Mouse, Rat WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) validation image for anti-Osteoactivin (GPNMB) (AA 81-180) antibody (ABIN749093)

IHC (fp)

Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (ABIN749093) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

SDS

Formalin-fixed and paraffin embedded rat lung labeled with Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC (p)

3 images

(6 references)

(1 validation)

Catalog No. ABIN749093

Datasheet as PDF

Target See all Osteoactivin (GPNMB) Antibodies

Osteoactivin (GPNMB)
Binding Specificity
14

8

7

6

5

4

2

2

1

1

1

1

1

1

1
AA 81-180
Reactivity
60

25

24

8

8

5

5

4

4

2

2

1
Human, Mouse, Rat
Host
47

14
Rabbit
Compatible Secondaries
Clonality
48

13
Polyclonal
Conjugate
21

4

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This Osteoactivin antibody is un-conjugated
Application
48

21

12

12

8

6

4

4

4

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Pig,Horse,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human GPNMB

Isotype

IgG
anti-Osteoactivin (GPNMB) (AA 31-260) antibody
GPNMB Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 7C10E5 unconjugated

anti-Osteoactivin (GPNMB) (AA 104-203) antibody
GPNMB Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1A8 unconjugated

anti-Osteoactivin (GPNMB) (N-Term) antibody
GPNMB Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Osteoactivin (GPNMB) (N-Term) antibody
GPNMB Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Osteoactivin (GPNMB) (AA 541-569), (C-Term) antibody
GPNMB Reactivity: Human WB Host: Rabbit Polyclonal RB36977 unconjugated

anti-Osteoactivin (GPNMB) (AA 104-203) antibody
GPNMB Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Osteoactivin (GPNMB) (AA 1-572) antibody
GPNMB Reactivity: Human WB, ELISA, IHC, IHC (p) Host: Mouse Monoclonal 3A5 unconjugated

anti-Osteoactivin (GPNMB) (AA 50-99) antibody
GPNMB Reactivity: Human, Mouse, Cow, Horse, Rabbit, Hamster WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Osteoactivin (GPNMB) (AA 1-572) antibody
GPNMB Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3A5 unconjugated

anti-Osteoactivin (GPNMB) (AA 74-123) antibody
GPNMB Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Dog, Rabbit, Zebrafish (Danio rerio), Hamster, Bat WB Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Validation #100002 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))

by

Memorial Sloan-Kettering Cancer Center, Parada lab

No.

#100002

Date

10/15/2015

Antigen

Osteoactivin

Lot Number

Method validated

Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Positive Control

Tumor cell transplanted mouse brain was dissected

Negative Control

Notes

The staining pattern was as expected for Osteoactivin as membrane protein.

Validation Images

IF validation of osteoactivin antibody ABIN749093 on mouse brain tumor sections. A. ABIN749093 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.
Full Methods
Primary Antibody

ABIN749093

Secondary Antibody

Donkey-anti-Rabbit-Cy3

Full Protocol

Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
Immunostaining for Cryo Sections:
Dry slides at 50-55°C for 15-30 min (or RT for 30min)
Wash three times with PBS 5min (shake gently)
Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
Rabbit-anti-Osteoactivin antibody (ABIN749093) diluted 1:50-1:400
Wash three times with PBS 5min (shake gently)
Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
Donkey-anti-Rabbit-Cy3
DAPI 1:100
Wash three times with PBS 5min (shake gently)
Add three drops of water-based Immu-mount
Cover with 24x60 cover glass
Imaging
Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.

Experimental Notes

ABIN749093 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Neal, Boyle, Budge, Safadi, Richardson: "The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 73, (2019) (PubMed).

Oyewumi, Manickavasagam, Novak, Wehrung, Paulic, Moussa, Sondag, Safadi: "Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells." in: Oncotarget, Vol. 7, Issue 12, pp. 13932-44, (2017) (PubMed).

Arosarena, Dela Cadena, Denny, Bryant, Barr, Thorpe, Safadi: "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." in: Journal of cellular physiology, Vol. 231, Issue 8, pp. 1761-70, (2016) (PubMed).

Frara, Abdelmagid, Tytell, Amin, Popoff, Safadi, Barbe: "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." in: BMC musculoskeletal disorders, Vol. 17, pp. 34, (2016) (PubMed).

Ruan, Li, Nie, Zhou, Zou: "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4857-66, (2015) (PubMed).

Abdelmagid, Sondag, Moussa, Belcher, Yu, Stinnett, Novak, Mbimba, Khol, Hankenson, Malcuit, Safadi: "Mutation in Osteoactivin Promotes Receptor Activator of NF?B Ligand (RANKL)-mediated Osteoclast Differentiation and Survival but Inhibits Osteoclast Function." in: The Journal of biological chemistry, Vol. 290, Issue 33, pp. 20128-46, (2015) (PubMed).
Target

Osteoactivin (GPNMB)

Alternative Name

GPNMB (GPNMB Products)

Background

Synonyms: NMB, HGFIN, Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, UNQ1725/PRO9925
Background: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene ID

10457

UniProt

Q14956