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MDC1 antibody

Name: MDC1 antibody
Brand: antibodies-online
SKU: ABIN2855454
Price: 609.18 USD
Availability: InStock

MDC1 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1, antibody at 1:500 dilution.

IHC

WB Image Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE antibody diluted at 1:1000

SMYD3 knockdown downregulates HR gene expressions.

3 images

(2 references)

Catalog No. ABIN2855454

Datasheet as PDF

Target See all MDC1 Antibodies

MDC1 (Mdc1 (MDC1))
Reactivity
59

54

16

12

11

2

1

1
Human
Host
49

37
Rabbit
Compatible Secondaries
Clonality
49

37
Polyclonal
Conjugate
29

6

6

4

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This MDC1 antibody is un-conjugated
Application
66

30

14

13

13

12

12

10

8

6

3

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Human

Characteristics

Rabbit Polyclonal antibody to MDC1 (mediator of DNA-damage checkpoint 1)
MDC1 antibody [N2N3]

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDC1. The exact sequence is proprietary.

Isotype

IgG
anti-Mdc1 (MDC1) (AA 1-100) antibody
MDC1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Mdc1 (MDC1) (AA 31-130) antibody
MDC1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Mdc1 (MDC1) (N-Term) antibody
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 unconjugated

anti-Mdc1 (MDC1) (N-Term) antibody (HRP)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 HRP

anti-Mdc1 (MDC1) (N-Term) antibody (FITC)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 FITC

anti-Mdc1 (MDC1) (N-Term) antibody (Alkaline Phosphatase (AP))
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 Alkaline Phosphatase (AP)

anti-Mdc1 (MDC1) (N-Term) antibody (Biotin)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 Biotin

anti-Mdc1 (MDC1) (N-Term) antibody (PE)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 PE

anti-Mdc1 (MDC1) (N-Term) antibody (PerCP)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 PerCP

anti-Mdc1 (MDC1) (N-Term) antibody (APC)
MDC1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal P2B11 APC

Application Notes

WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: HeLa nucleus
Validation: Orthogonal

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Burger, Schlackow, Gullerova: "Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks." in: Nucleic acids research, Vol. 47, Issue 7, pp. 3467-3484, (2019) (PubMed).

Chen, Tsai, Wang, Teng: "SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression." in: Scientific reports, Vol. 7, Issue 1, pp. 3842, (2017) (PubMed).
Target

MDC1 (Mdc1 (MDC1))

Alternative Name

mediator of DNA damage checkpoint 1 (MDC1 Products)

Synonyms

6820401C03 antibody, AA413496 antibody, Nfbd1 antibody, mKIAA0170 antibody, NFBD1 antibody, mediator of DNA damage checkpoint 1 antibody, mediator of DNA-damage checkpoint 1 antibody, Mdc1 antibody, MDC1 antibody

Background

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Cellular Localization: Nucleus

Molecular Weight

227 kDa

Gene ID

9656

UniProt

Q14676