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CDC25A antibody (pSer124)

Name: CDC25A antibody (pSer124)
SKU: ABIN389527
Price: 429 USD
Availability: InStock

CDC25A Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB07634 unconjugated

Western blot analysis of lysates from cell line, untreated or treated with Alkaline phosphatase, 1h, using 459085101 A (upper) or Beta-actin (lower).

Immunohistochemical analysis of A on paraffin-embedded Human breast carcinoma tissue.

IHC (p)

Western blot analysis of lysates from cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088101 A (upper) or Beta-actin (lower).

Western blot analysis of lysates from 293 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088102 A (upper) or Beta-actin (lower).

The anti-phospho CDC25A Pab (ABIN389527 and ABIN2839576) is used in Western blot to detect CDC25A in mouse liver tissue lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

IHC (p)

6 images

(2 references)

Catalog No. ABIN389527

Datasheet as PDF

Target See all CDC25A Antibodies

CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
Binding Specificity
33

33

21

17

15

12

9

9

8

8

7

7

7

6

6

5

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer124
Reactivity
234

93

77

5

4

4

4

4

2
Human
Host
243

7
Rabbit
Compatible Secondaries
Clonality
244

6
Polyclonal
Conjugate
107

21

16

15

11

11

9

9

9

9

9

9

3

3

3

3

3
This CDC25A antibody is un-conjugated
Application
192

124

69

40

40

31

21

17

15

8

7

6

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

Rat

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC25A.

Clone

RB07634

Isotype

Ig Fraction
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 235-484) antibody
CDC25A Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer75) antibody
CDC25A Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibody
CDC25A Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer178) antibody
CDC25A Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody
CDC25A Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibody
CDC25A Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 480-514), (pThr507) antibody
CDC25A Reactivity: Human WB, IHC (p), IF Host: Rabbit Polyclonal RB7856 unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) antibody
CDC25A Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 1-524) antibody
CDC25A Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) antibody
CDC25A Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500. WB: 1:500. WB: 1:500. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:50~100

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Zoppoli, Solier, Reinhold, Liu, Connelly, Monks, Shoemaker, Abaan, Davis, Meltzer, Doroshow, Pommier: "CHEK2 genomic and proteomic analyses reveal genetic inactivation or endogenous activation across the 60 cell lines of the US National Cancer Institute." in: Oncogene, Vol. 31, Issue 4, pp. 403-18, (2012) (PubMed).

Solier, Sordet, Kohn, Pommier: "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways." in: Molecular and cellular biology, Vol. 29, Issue 1, pp. 68-82, (2008) (PubMed).
Target

CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name

CDC25A (CDC25A Products)

Synonyms

cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight

59087

Gene ID

993

NCBI Accession

NP_001780, NP_963861

UniProt

P30304

Pathways

Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase