β-Glucosyltransferase enzyme

Details for Product No. ABIN2669404, Supplier: Log in to see
Antigen
Reactivity
Bacteriophage T4
Host
Escherichia coli (E. coli)
Application
Activity Assay (AcA), Tet-assisted Bisulfite Sequencing (TAB-Seq)
Options
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Characteristics β-Glucosyltransferase will transfer the glucose moiety from the UDP-Glucose (uridine diphosphoglucose) to 5-hydroxymethylcytosine residues in double-stranded DNA.
Components 200,000 Units β-Glucosyltransferase enzyme
10X β-Glucosyltransferase Reaction Buffer
50 mM UDP-Glucose
1 M DTT
Application Notes The β-Glucosyltransferase enzyme can be used to glucosylate 5-hmC DNA. Glucosylated DNA can be used as a differentiator with glucosyl-sensitive restriction enzymes. The use of a labeled [14C] UDP-Glucose donor would enable labeling of the 5-hmC residues.
Comment

One unit is defined as the amount of enzyme required to fully protect 0.5 µg of 5-hmC DNA Standard (Catalog No. 55008) in 1 hour at 37°C in a total reaction volume of 50 µl from cleavage by 4 units of GlaI restriction enzyme in an incubation of 16 hours at 30°C. A pilot titration of enzyme is recommended for protection of genomic DNA.

Restrictions For Research Use only
Storage -20 °C/-80 °C
Storage Comment Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Supplier Images
Restriction Digest (RD) image for β-Glucosyltransferase enzyme (ABIN2669404) Figure 1: 0.5 µg of a 5-hmC Methylated DNA Standard (Catalog No. 55008) was incubated...