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These findings suggest that the targets of AGO4-directed RNA-directed DNA methylation (show HELLS ELISA Kits) are regulatory units responsible for controlling gene expression under specific environmental conditions.
AGO4 and AGO6 mainly act sequentially in mediating small RNA-directed DNA methylation (show HELLS ELISA Kits).
Trans-acting small interfering RNAs are associated with AGO4 proteins to direct DNA methylation (show HELLS ELISA Kits).
selective nuclear import of mature AGO4/siRNA complexes is a key regulatory point prior to the effector stage of RdDM.
DNA-dependent RNA polymerase IV-dependent smRNAs were mainly 24 nt and associated with AGO4.
our data suggest that AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent RNA Pol V transcripts.
Our results suggest a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci.
AGO4 binds to small RNAs including small interfering RNAs (siRNAs) originating from transposable and repetitive elements, and cleaves target RNA transcripts
AGO4 was partially compromised in the reception of silencing.
OCP11 was cloned and shown that it encodes ARGONAUTE4 (AGO4), a component of the pathway that mediates the transcriptional gene silencing associated with small interfering RNAs that direct DNA methylation (show HELLS ELISA Kits) at specific loci.
Chimeras with Ago4 N-terminal domains were defective in RISC (show SCPEP1 ELISA Kits) activation & sometimes also in RNA loading. This highlights the pleiotropic role of the Ago N domain & emphasizes the unique position of human Ago4 within the Ago protein family.
EIF2C2 (show EIF2C2 ELISA Kits)-4 and PIWIL4 (show PIWIL4 ELISA Kits) appear increased in advanced tumors with distant metastasis, suggesting they may promote tumor invasion
The specificity of RNA interference depends on the concentration of Ago1 (show EIF2C1 ELISA Kits), Ago3 (show EIF2C3 ELISA Kits), and Ago4 relative to Ago2 (show EIF2C2 ELISA Kits).
EIF2C4 protein is expressed in differentiating and terminally differentiated N-type cells more than in S- and I-type cells
up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR (show MLXIP ELISA Kits)-71
show that Ago4(-/-) spermatogonia initiate meiosis early, resulting from premature induction of retinoic acid-response genes
Data show that Ago4- Ago1 (show EIF2C1 ELISA Kits)-Ago3 (show EIF2C3 ELISA Kits) genes are linked together at the p12 (show SPRN ELISA Kits) of the chromosome 6, while Ago2 (show EIF2C2 ELISA Kits) is located at the p15 (show CDKN2B ELISA Kits) of the chromosome 4.
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.
eukaryotic translation initiation factor 2C, 4
, argonaute 4
, protein argonaute-4
, eIF-2C 4
, eIF2C 4
, Piwi/Argonaute family protein meIF2C4
, argonaute RISC catalytic component 4