Mouse (Murine) FGF1 ELISA Kit

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Antigen
Fibroblast Growth Factor 1 (Acidic) (FGF1) ELISA Kits
  • FGF1
  • zgc:136885
  • aFGF
  • ecgf
  • fgfa
  • ecgfa
  • ecgfb
  • fgf-1
  • hbgf1
  • HBGF-1
  • glio703
  • ecgf-beta
  • fgf-alpha
  • LOC100221393
  • FGF-1
  • Fgf1
  • Dffrx
  • Fam
  • Fgf-1
  • Fgfa
  • ECGF
  • AFGF
  • ECGF-beta
  • ECGFA
  • ECGFB
  • FGF-alpha
  • FGFA
  • GLIO703
  • HBGF1
  • fibroblast growth factor 1 (acidic)
  • fibroblast growth factor 1b
  • acidic fibroblast growth factor
  • fibroblast growth factor 1
  • fgf1
  • FGF1
  • fgf1b
  • LOC100221393
  • AFGF
  • Fgf1
Epitope
AA 16-155
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
27
23
21
13
12
12
10
5
4
3
3
3
2
1
1
Method Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1889349
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN415590 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
10.090268 ABIN4885416 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 12-3000 pg/mL 12 pg/mL Log in to see
10.090268 ABIN2708506 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN365481 Sandwich ELISA Serum, Plasma, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see
1 ABIN1569782 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN627551 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN1081402 Sandwich ELISA Serum, Plasma, Biological Fluids 2.74-2.000 pg/mL 2.74 pg/mL Log in to see
1 ABIN4992998 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 12.3-3000 pg/mL 12.3 pg/mL Log in to see
1 ABIN1884730 Sandwich ELISA Plasma, Serum, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4947556 Sandwich ELISA Log in to see
1 ABIN5654181 Sandwich ELISA Biological Fluids, Plasma, Serum 31.25 pg/mL - 2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN5654182 Sandwich ELISA Biological Fluids, Plasma, Serum 15.62 pg/mL - 1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN509764 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
1 ABIN5519789 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN5654172 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 12.3 pg/mL - 3000 pg/mL 12.3 pg/mL Log in to see
1 ABIN4885692 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see
1 ABIN2684253 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN4969083 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2533365 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
1 ABIN454112 31.2-2000 pg/mL 31.2 pg/mL Log in to see

General

Antigen Fibroblast Growth Factor 1 (Acidic) (FGF1) ELISA Kits
Epitope AA 16-155
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(27), (23), (21), (13), (12), (12), (10), (5), (4), (3), (3), (3), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details FGF1 ELISA Kit

Target details Application Details Handling References for FGF1 Kit (ABIN1889349) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FGF1
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: E.coli
Immunogen sequence: F16-D155
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: F16-D155

Target details

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Antigen
Alternative Name FGF1 (FGF1 ELISA Kit Abstract)
Background

Protein Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity). .

Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

Synonyms: Fibroblast growth factor 1,FGF-1,Acidic fibroblast growth factor,aFGF,Heparin-binding growth factor 1,HBGF-1,Fgf1,Fgf-1, Fgfa,

Full Gene Name: Fibroblast growth factor 1

Cellular Localisation: Secreted. Cytoplasm . Cytoplasm, cell cortex . Cytoplasm, cytosol . Nucleus . Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity)..
Gene ID 14164
UniProt P61148
Research Area Cancer, Angiogenesis, Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details FGF1 ELISA Kit Target details Handling References for FGF1 Kit (ABIN1889349) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol mouse FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF1 has been precoated onto 96-well plates. Standards(E.coli, F16-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 158, Standard deviation: 8.53, CV(%): 5.4
  • Sample 2: n=16, Mean(pg/ml): 754, Standard deviation: 58.81, CV(%): 7.8
  • Sample 3: n=16, Mean(pg/ml): 1219, Standard deviation: 41.45, CV(%): 3.4,
  • Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 13.98, CV(%): 9.2
  • Sample 2: n=24, Mean(pg/ml): 747, Standard deviation: 63.50, CV(%): 8.5
  • Sample 3: n=24, Mean(pg/ml): 1223, Standard deviation: 111.3, CV(%): 9.1
Restrictions For Research Use only

Handling

Product Details FGF1 ELISA Kit Target details Application Details References for FGF1 Kit (ABIN1889349) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FGF1 Kit (ABIN1889349)

Product Details FGF1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Dionne, Crumley, Bellot, Kaplow, Searfoss, Ruta, Burgess, Jaye, Schlessinger: "Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors." in: The EMBO journal, Vol. 9, Issue 9, pp. 2685-92, 1990

Jaye, Howk, Burgess, Ricca, Chiu, Ravera, OBrien, Modi, Maciag, Drohan: "Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization." in: Science (New York, N.Y.), Vol. 233, Issue 4763, pp. 541-5, 1986

Images

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Supplier Images
ELISA image for Fibroblast Growth Factor 1 (Acidic) (FGF1) ELISA Kit (ABIN1889349) Mouse FGF1 PicoKine ELISA Kit standard curve