Caspase-10 Inhibitor AEVD-FMK

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Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-10 inhibitor
Sequence Z-Ala-Glu(OMe)-Val-Asp(OMe)-FMK
Characteristics PubChem CID: 44135225
A synthetic peptide inhibitor that inhibits caspase-10 and related caspase activity. FMK inhibitors are irreversible and non-toxic, and can be used in both in vivo and in vitro studies. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity > 95 % by HPLC
Chemical Name Z-AEVD-FMK, Caspase-10 Inhibitor (fluoromethylketone)
Formula C₂₈H₃₉N₄O₁₀F
Permeability Cell-permeable
Solubility In DMSO (10 mM)
Molecular Weight 610.63 g/mol
Application Notes We recommend using 1000-5000X dilutions for inhibiting caspase-10 and related caspase activity (e.g., Add 1 µL to 1-5 mL of culture medium). The optimal doses may vary for different cells and culture conditions. The inhibitor is designed as a methyl ester to facilitate cell permeability.
Restrictions For Research Use only
Format Liquid
Concentration 10 mM
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Product cited in: Appelqvist, Wäster, Eriksson, Rosdahl, Ollinger: "Lysosomal exocytosis and caspase-8-mediated apoptosis in UVA-irradiated keratinocytes." in: Journal of cell science, Vol. 126, Issue Pt 24, pp. 5578-84, 2013 (PubMed).

Westphal, Sytnyk, Schachner, Leshchynska: "Clustering of the neural cell adhesion molecule (NCAM) at the neuronal cell surface induces caspase-8- and -3-dependent changes of the spectrin meshwork required for NCAM-mediated neurite outgrowth." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 42046-57, 2010 (PubMed).

Klumpp, Rycyk, Chen, Thumbikat, Sengupta, Schaeffer: "Uropathogenic Escherichia coli induces extrinsic and intrinsic cascades to initiate urothelial apoptosis." in: Infection and immunity, Vol. 74, Issue 9, pp. 5106-13, 2006 (PubMed).

Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Ruckdeschel, Mannel, Schröttner: "Divergence of apoptosis-inducing and preventing signals in bacteria-faced macrophages through myeloid differentiation factor 88 and IL-1 receptor-associated kinase members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 9, pp. 4601-11, 2002 (PubMed).

Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).