Z-VAD-FMK

Details for Product No. ABIN412070, Supplier: Log in to see
Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A pan-caspase inhibitor
Sequence Z-Val-Ala-Asp(OMe)-FMK
Characteristics A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity Single spot by TLC
Chemical Name Z-VAD-FMK, Z-VAD-fluoromethylketone, Caspase-Family Inhibitor (fluoromethylketone)
Formula C₂₂H₃₀N₃O₇F
Permeability Cell-permeable
Solubility DMSO (20 mM)
Molecular Weight 467.5 g/mol
CAS-No 187389-52-2
Application Notes We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Solid, Lyophilized
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Supplier Images
 image for Z-VAD-FMK (ABIN412070) Z-VAD-FMK
Product cited in: Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014 (PubMed).

Robinson, Shridar, Smith, Murrell-Lagnado: "Plasma membrane cholesterol as a regulator of human and rodent P2X7 receptor activation and sensitization." in: The Journal of biological chemistry, Vol. 289, Issue 46, pp. 31983-94, 2014 (PubMed).

Wang, Wang, Zhou, Wei: "Farnesol induces apoptosis-like cell death in the pathogenic fungus Aspergillus flavus." in: Mycologia, Vol. 106, Issue 5, pp. 881-8, 2014 (PubMed).

Hashimoto, Todo: "Mitotic slippage underlies the relationship between p53 dysfunction and the induction of large micronuclei by colcemid." in: Mutagenesis, Vol. 28, Issue 4, pp. 457-64, 2013 (PubMed).

Saha, Jiang, Yang, Reece, Chang: "PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa." in: Molecular cancer therapeutics, Vol. 12, Issue 11, pp. 2331-41, 2013 (PubMed).

Breinig, Mayer, Harjung, Goeppert, Malz, Penzel, Neumann, Hartmann, Dienemann, Giaccone, Schirmacher, Kern, Chiosis, Rieker: "Heat shock protein 90-sheltered overexpression of insulin-like growth factor 1 receptor contributes to malignancy of thymic epithelial tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 8, pp. 2237-49, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011 (PubMed).

Xie, Jiang, Wan, Du, Guo, Liu, Ye, Niu, Wu, Dong, Zhang: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011 (PubMed).

Dolai, Pal, Yadav, Adak: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed).

Ariyama, Kono, Matsuda, Inoue, Arai: "Decrease in membrane phospholipid unsaturation induces unfolded protein response." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22027-35, 2010 (PubMed).

Fukazawa, Maeda, Matsuoka, Yamatsuji, Shigemitsu, Morita, Faiola, Durbin, Soucek, Naomoto: "Inhibition of Myc effectively targets KRAS mutation-positive lung cancer expressing high levels of Myc." in: Anticancer research, Vol. 30, Issue 10, pp. 4193-200, 2010 (PubMed).

Gao, Bauvy, Souquère, Tonelli, Liu, Zhu, Qiao, Bakula, Proikas-Cezanne, Pierron, Codogno, Chen, Mehrpour: "The Bcl-2 homology domain 3 mimetic gossypol induces both Beclin 1-dependent and Beclin 1-independent cytoprotective autophagy in cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25570-81, 2010 (PubMed).

Saha, Jiang, Mukai, Chang: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010 (PubMed).

Ito, Yip, Katz, Fonseca, Hedley, Chow, Xu, Wood, Bastianutto, Schimmer, Kelley, Liu: "Potential use of cetrimonium bromide as an apoptosis-promoting anticancer agent for head and neck cancer." in: Molecular pharmacology, Vol. 76, Issue 5, pp. 969-83, 2009 (PubMed).

Liu, Deng, Lancto, Abrahamsen, Rutherford, Enomoto: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed).

Dai, Liu, Tang, DeSano, Burstein, Davis, Pienta, Lawrence, Xu: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed).

Liu, Enomoto, Lancto, Abrahamsen, Rutherford: "Inhibition of apoptosis in Cryptosporidium parvum-infected intestinal epithelial cells is dependent on survivin." in: Infection and immunity, Vol. 76, Issue 8, pp. 3784-92, 2008 (PubMed).

Upton, Austgen, Nishino, Coakley, Hagen, Han, Papa, Oakes: "Caspase-2 cleavage of BID is a critical apoptotic signal downstream of endoplasmic reticulum stress." in: Molecular and cellular biology, Vol. 28, Issue 12, pp. 3943-51, 2008 (PubMed).

Niikura, Dixit, Scott, Perkins, Kitagawa: "BUB1 mediation of caspase-independent mitotic death determines cell fate." in: The Journal of cell biology, Vol. 178, Issue 2, pp. 283-96, 2007 (PubMed).