You are viewing an incomplete version of our website. Please click to reload the website as full version.

Z-IETD-FMK (Caspase-8 Inhibitor)

Details for Product No. ABIN412083, Supplier: Log in to see
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
Log in to see
Supplier Product No.
Log in to see

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Purpose A caspase-8 inhibitor
Sequence Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK
Characteristics A synthetic peptide that irreversibly inhibits FLICE and related protease/caspase activity and blocks apoptosis. IETD is the specific recognition sequence found in CPP32 proenzyme. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups.)
Purity Single spot by TLC
Chemical Name Z-IETD-fluoromethylketone
Formula C₃₀H₄₃FN₄O₁₁
Permeability Cell-permeable
Solubility DMSO (20 mM)
Molecular Weight 654.7 Da
Application Notes We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary among different cells and culture conditions.
Restrictions For Research Use only
Format Lyophilized
Handling Advice Protect from light and moisture.
Storage -20 °C
Expiry Date 12 months
Supplier Images
 image for Z-IETD-FMK (Caspase-8 Inhibitor) (ABIN412083) Z-IETD-FMK (Caspase-8 Inhibitor)
Product cited in: Liang, Wang, Chen: "Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 9, pp. 1354-63, 2014

Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011

Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011

Du, Yamamoto, Ueda, Suzuki, Tano, Kamei: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011

Abdul-Ghani, Dufort, Stiles, De Repentigny, Kothary, Megeney: "Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals." in: Molecular and cellular biology, Vol. 31, Issue 1, pp. 163-78, 2010

Saha, Jiang, Mukai, Chang: "RITA inhibits multiple myeloma cell growth through induction of p53-mediated caspase-dependent apoptosis and synergistically enhances nutlin-induced cytotoxic responses." in: Molecular cancer therapeutics, Vol. 9, Issue 11, pp. 3041-51, 2010

Tang, Liu, Wang, Wang, Cao, Mi, Zhang: "Involvement of caspase 8 in apoptosis induced by ultrasound-activated hematoporphyrin in sarcoma 180 cells in vitro." in: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, Vol. 27, Issue 4, pp. 645-56, 2008