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Z-LEHD-FMK (Caspase-9 Inhibitor)

Details for Product No. ABIN412084, Supplier: Log in to see
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-9 inhibitor
Sequence Z-Leu-Glu(OMe)-His-Asp(OMe)-FMK.TFA
Characteristics A synthetic peptide that irreversibly inhibits caspase-9 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity Single spot by TLC
Chemical Name Z-LEHD-fluoromethylketone
Formula C₃₂H₄₃FN₆O₁₀
Permeability Cell-permeable
Solubility DMSO (20 mM)
Molecular Weight 690.71 Da
Application Notes We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary for different cells and culture conditions.
Note: For research purpose only! Not to be used in humans.
Restrictions For Research Use only
Format Lyophilized
Handling Advice Protect from light and moisture.
Storage -20 °C
Expiry Date 12 months
Product cited in: Kim, Amarnani, Gnanaguru, Tseng, Vavvas, DAmore: "Tamoxifen toxicity in cultured retinal pigment epithelial cells is mediated by concurrent regulated cell death mechanisms." in: Investigative ophthalmology & visual science, Vol. 55, Issue 8, pp. 4747-58, 2014

Pianu, Lefort, Thuiliere, Tabourier, Bartolini: "The Aβ₁₋₄₂ peptide regulates microtubule stability independently of tau." in: Journal of cell science, Vol. 127, Issue Pt 5, pp. 1117-27, 2014

Du, Yamamoto, Ueda, Suzuki, Tano, Kamei: "Activated protein C rescues the retina from ischemia-induced cell death." in: Investigative ophthalmology & visual science, Vol. 52, Issue 2, pp. 987-93, 2011

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011

Hara, Senga, Biswas, Hasegawa, Ito, Hyodo, Hirooka, Niwa, Goto, Hamaguchi: "Recovery of anoikis in Src-transformed cells and human breast carcinoma cells by restoration of the SIRP α1/SHP-2 signaling system." in: Cancer research, Vol. 71, Issue 4, pp. 1229-34, 2011

Verma, Jain, Caseltine, Rennels, Bhattacharya, Markiewski, Rawat, Neethling, Bickel, Weidanz: "TCR mimic monoclonal antibodies induce apoptosis of tumor cells via immune effector-independent mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 3265-76, 2011

Xie, Jiang, Wan, Du, Guo, Liu, Ye, Niu, Wu, Dong, Zhang: "Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway." in: Journal of molecular cell biology, Vol. 3, Issue 4, pp. 250-9, 2011

Lipke, Matute-Bello, Herrero, Kurahashi, Wong, Mongovin, Martin: "Febrile-range hyperthermia augments lipopolysaccharide-induced lung injury by a mechanism of enhanced alveolar epithelial apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3801-13, 2010

Bian, Elner, Elner: "Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 50, Issue 12, pp. 6006-14, 2009

Jin, Ojcius, Sun, Dong, Luo, Mao, Yan: "Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways." in: Infection and immunity, Vol. 77, Issue 2, pp. 799-809, 2009