Z-ATAD-FMK

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Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-12 inhibitor
Sequence Z-Ala-Thr-Ala-Asp(OMe)-FMK
Characteristics A synthetic peptide that irreversibly inhibits caspase-12 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity Single spot by TLC
Chemical Name Z-ATAD-FMK, Z-ATAD-fluoromethylketone, Caspase-12 Inhibitor (fluoromethylketone)
Formula C₂₄H₃₃FN₄O₉
Permeability Cell-permeable
Solubility DMSO (20 mM)
Molecular Weight 540.54 g/mol
Application Notes We recommend using 2-10 µM for inhibiting caspase activity in cell culture.
The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Solid, Lyophilized
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Product cited in: Dolai, Pal, Yadav, Adak: "Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13638-46, 2011 (PubMed).

Ariazi, Cunliffe, Lewis-Wambi, Slifker, Willis, Ramos, Tapia, Kim, Yerrum, Sharma, Nicolas, Balagurunathan, Ross, Jordan: "Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. 18879-86, 2011 (PubMed).

Suzuki, Lee, Matsuoka: "TDP-43-induced death is associated with altered regulation of BIM and Bcl-xL and attenuated by caspase-mediated TDP-43 cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13171-83, 2011 (PubMed).

Auner, Beham-Schmid, Dillon, Sabbattini: "The life span of short-lived plasma cells is partly determined by a block on activation of apoptotic caspases acting in combination with endoplasmic reticulum stress." in: Blood, Vol. 116, Issue 18, pp. 3445-55, 2010 (PubMed).

Fu, Minamino, Tsukamoto, Sawada, Asai, Kato, Asano, Fujita, Takashima, Hori, Kitakaze: "Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition." in: Cardiovascular research, Vol. 79, Issue 4, pp. 600-10, 2008 (PubMed).

Ou, Wu, Ip, Meng, Hsu, Ip: "Apoptosis induced by t10,c12-conjugated linoleic acid is mediated by an atypical endoplasmic reticulum stress response." in: Journal of lipid research, Vol. 49, Issue 5, pp. 985-94, 2008 (PubMed).

Trisciuoglio, Uranchimeg, Cardellina, Meragelman, Matsunaga, Fusetani, Del Bufalo, Shoemaker, Melillo: "Induction of apoptosis in human cancer cells by candidaspongiolide, a novel sponge polyketide." in: Journal of the National Cancer Institute, Vol. 100, Issue 17, pp. 1233-46, 2008 (PubMed).