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Z-DEVD-FMK (Caspase-3 Inhibitor)

Details for Product No. ABIN412345, Supplier: Log in to see
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-3 inhibitor
Sequence Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
Characteristics PubChem CID: 16760394
A synthetic peptide that irreversibly inhibits activity of DEVD-dependent caspases (e.g., caspase-3 and caspase-7) and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity > 98 % by HPLC
Chemical Name Z-DEVD fluoromethylketone
Formula C₃₀H₄₁FN₄O₁₂
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 668.7 Da
Application Notes We recommend using 1000-5000X dilutions for inhibiting caspase-3 activity in Jurkat cell culture (e.g., Add 1 µL to 1-5 mL of culture medium). The inhibitor can be added t°Culture medium together with apoptosis inducer or 1 hr before inducer is added. The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Liquid
Concentration 10 mM
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 6-12 months
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 image for Z-DEVD-FMK (Caspase-3 Inhibitor) (ABIN412345) Z-DEVD-FMK (Caspase-3 Inhibitor)
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Nair, McNaught, González-Maeso et al.: "p53 mediates nontranscriptional cell death in dopaminergic cells in response to proteasome inhibition." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39550-60, 2006 (PubMed).

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Lin, Cheung, Wu et al.: "15d-prostaglandin J2 protects brain from ischemia-reperfusion injury." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 481-7, 2006 (PubMed).

Mao, Iwai, Keng et al.: "Norepinephrine-induced oxidative stress causes PC-12 cell apoptosis by both endoplasmic reticulum stress and mitochondrial intrinsic pathway: inhibition of phosphatidylinositol 3-kinase survival pathway." in: American journal of physiology. Cell physiology, Vol. 290, Issue 5, pp. C1373-84, 2006 (PubMed).

Gillet, Dewals, Farnir et al.: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Bi, Stuelten, Kilts et al.: "Extracellular matrix proteoglycans control the fate of bone marrow stromal cells." in: The Journal of biological chemistry, Vol. 280, Issue 34, pp. 30481-9, 2005 (PubMed).

Kubota, Lee, Tsuchiya et al.: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).

Miura, Chen, Allen et al.: "A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells." in: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1704-13, 2004 (PubMed).

Wang, Grégori, Hullinger et al.: "Sustained activation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase pathways by hepatitis B virus X protein mediates apoptosis via induction of Fas/FasL and tumor necrosis factor ..." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10352-65, 2004 (PubMed).

Song, Zhou, Kim et al.: "Carbon monoxide promotes Fas/CD95-induced apoptosis in Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44327-34, 2004 (PubMed).

Song, Mahidhara, Zhou et al.: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Yen, Mar, Craciunescu et al.: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).