Caspase-Family Inhibitor Z-VAD-FMK

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Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A pan-caspase inhibitor
Sequence Z-Val-Ala-Asp(OMe)-FMK
Characteristics PubChem CID: 5737
A synthetic peptide that irreversibly inhibits activity of caspase family proteases and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability. The product can be used for both in vitro and in vivo studies. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity Single spot by TLC
Chemical Name Z-VAD-FMK, Z-VAD fluoromethylketone, Caspase-Family Inhibitor, pan-caspase inhibitor
Formula C₂₂H₃₀N₃O₇F
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 467.5 g/mol
CAS-No 187389-52-2
Application Notes We recommend using 1000X dilutions for inhibiting caspase activity in Jurkat cell culture (e.g., Add 1 µL to 1 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Liquid
Concentration 10 mM
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 6-12 months
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 image for Caspase-Family Inhibitor Z-VAD-FMK (ABIN412347) Caspase-Family Inhibitor Z-VAD-FMK
Product cited in: Jouve, Despoix, Espeli, Gauthier, Cypowyj, Fallague, Schiff, Dignat-George, Vély, Leroyer: "The involvement of CD146 and its novel ligand Galectin-1 in apoptotic regulation of endothelial cells." in: The Journal of biological chemistry, Vol. 288, Issue 4, pp. 2571-9, 2013 (PubMed).

Liu, Deng, Lancto, Abrahamsen, Rutherford, Enomoto: "Biphasic modulation of apoptotic pathways in Cryptosporidium parvum-infected human intestinal epithelial cells." in: Infection and immunity, Vol. 77, Issue 2, pp. 837-49, 2009 (PubMed).

Dai, Liu, Tang, DeSano, Burstein, Davis, Pienta, Lawrence, Xu: "Molecularly targeted radiosensitization of human prostate cancer by modulating inhibitor of apoptosis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 23, pp. 7701-10, 2008 (PubMed).

Liu, Enomoto, Lancto, Abrahamsen, Rutherford: "Inhibition of apoptosis in Cryptosporidium parvum-infected intestinal epithelial cells is dependent on survivin." in: Infection and immunity, Vol. 76, Issue 8, pp. 3784-92, 2008 (PubMed).

Arai, Miyake, Voit, Nemoto, Wakeland, Grummt, Miyazaki: "Death-effector domain-containing protein DEDD is an inhibitor of mitotic Cdk1/cyclin B1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2289-94, 2007 (PubMed).

Niikura, Dixit, Scott, Perkins, Kitagawa: "BUB1 mediation of caspase-independent mitotic death determines cell fate." in: The Journal of cell biology, Vol. 178, Issue 2, pp. 283-96, 2007 (PubMed).

Deng, Yin, Lu, Cai, Yin: "The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 91, Issue 1, pp. 59-69, 2006 (PubMed).

Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Pae, Choi, Oh, Lee, Ryu, Rhew, Kim, Chung: "Roles of heme oxygenase-1 in the antiproliferative and antiapoptotic effects of nitric oxide on Jurkat T cells." in: Molecular pharmacology, Vol. 66, Issue 1, pp. 122-8, 2004 (PubMed).

Song, Mahidhara, Zhou, Hoffman, Seol, Flavell, Billiar, Otterbein, Choi: "Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1220-6, 2004 (PubMed).

Fukazawa, Walter, Owen-Schaub: "Adenoviral Bid overexpression induces caspase-dependent cleavage of truncated Bid and p53-independent apoptosis in human non-small cell lung cancers." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25428-34, 2003 (PubMed).

Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).

Erdreich-Epstein, Tran, Bowman, Wang, Cabot, Durden, Vlckova, Reynolds, Stins, Groshen, Millard: "Ceramide signaling in fenretinide-induced endothelial cell apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49531-7, 2002 (PubMed).