Caspase-6 Inhibitor Z-VEID-FMK

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Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-4 inhibitor
Sequence Z-Val-E(OMe)-Ile-Asp(OMe)-FMK
Characteristics PubChem CID: 44135214
VEID is the specific recognition sequence for caspase-6/Mch2. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity > 98 % by HPLC
Chemical Name Z-VEID-FMK, Caspase-6 Inhibitor, VEID-FMK, VEID fluoromethylketone
Formula C₃₁H₄₅FN₄O₁₀
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 652.9 g/mol
Application Notes We recommend using 1000-5000X dilutions for inhibiting caspase-6 activity in Jurkat cell culture (e.g., Add 1 µL to 1-5 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Liquid
Concentration 10 mM
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 6-12 months
Product cited in: Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Pelizon, dAdda di Fagagna, Farrace, Laskey: "Human replication protein Cdc6 is selectively cleaved by caspase 3 during apoptosis." in: EMBO reports, Vol. 3, Issue 8, pp. 780-4, 2002 (PubMed).

Yen, Mar, Craciunescu, Edwards, Zeisel: "Deficiency in methionine, tryptophan, isoleucine, or choline induces apoptosis in cultured cells." in: The Journal of nutrition, Vol. 132, Issue 7, pp. 1840-7, 2002 (PubMed).