Etoposide (100 mM)

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Application
Functional Studies (Func), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A topoisomerase II inhibitor
Characteristics Etoposide is an antitumor agent that complexes with topoisomerase II and DNA to enhance double-strand and single-strand cleavage of DNA and reversibly inhibit religation. Blocks the cell cycle in in S-phase and G2-phase of the cell cycle, induces apoptosis in normal and tumor cell lines, inhibits synthesis of the oncoprotein Mdm2 and induces apoptosis in tumor lines that overexpress Mdm2.
Purity > 98 % by HPLC
Chemical Name 4'-Demethylepipodophyllotoxin 9-(4,6-O-ethylidene-β-D-glucopyranoside, VP-16-213
Formula C₂₂H₂₉FO₅
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 588.56 g/mol
CAS-No 33419-42-0
Application Notes We recommend using 1000X dilutions for inducing apoptosis in Jurkat cells (e.g., Add 1 µL to 1 mL of culture medium).
However the optimal doses may vary for different cells and culture conditions.
Comment

A topoisomerase II inhibitor. A derivative of podophyllotoxin that has major activity against a number of tumors, including germ cell neoplasms, small cell lung cancer, and malignant lymphoma. Induces apoptosis in human T cells, mouse thymocytes, and HL-60 human leukemia cells

Restrictions For Research Use only
Format Liquid
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 6 months
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 image for Etoposide (100 mM) (ABIN412366) Etoposide (100 mM)
Product cited in: Chen, Colvin, Maier, Mirmira, Fueger: "Mitogen-inducible gene 6 triggers apoptosis and exacerbates ER stress-induced β-cell death." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 1, pp. 162-71, 2012 (PubMed).

Itamochi, Oishi, Shimada, Sato, Uegaki, Naniwa, Sato, Nonaka, Terakawa, Kigawa, Harada: "Inhibiting the mTOR pathway synergistically enhances cytotoxicity in ovarian cancer cells induced by etoposide through upregulation of c-Jun." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 14, pp. 4742-50, 2011 (PubMed).

Xia, Ma, Sun, Yang, Peng: "Retinoic acid metabolizing enzyme CYP26A1 is implicated in rat embryo implantation." in: Human reproduction (Oxford, England), Vol. 25, Issue 12, pp. 2985-98, 2010 (PubMed).

Bhola, Simon: "Determinism and divergence of apoptosis susceptibility in mammalian cells." in: Journal of cell science, Vol. 122, Issue Pt 23, pp. 4296-302, 2009 (PubMed).

Jensen, Andresen, Hansen, Skov: "Cell-surface expression of Hsp70 on hematopoietic cancer cells after inhibition of HDAC activity." in: Journal of leukocyte biology, Vol. 86, Issue 4, pp. 923-32, 2009 (PubMed).

Lin, Chen, Chang, Jan, Hsu, Wu, Fang, Tang, Chang, Lin: "Bcl-2 rescues ceramide- and etoposide-induced mitochondrial apoptosis through blockage of caspase-2 activation." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23758-65, 2005 (PubMed).