Etoposide (100 mM)

Details for Product No. ABIN412366, Supplier: Log in to see
Functional Studies (Func), Blocking Reagent (BR), Inhibition Assay (InhA)
Log in to see
Supplier Product No.
Log in to see

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Purpose A topoisomerase II inhibitor
Characteristics Etoposide is an antitumor agent that complexes with topoisomerase II and DNA to enhance double-strand and single-strand cleavage of DNA and reversibly inhibit religation. Blocks the cell cycle in in S-phase and G2-phase of the cell cycle, induces apoptosis in normal and tumor cell lines, inhibits synthesis of the oncoprotein Mdm2 and induces apoptosis in tumor lines that overexpress Mdm2.
Purity > 98 % by HPLC
Chemical Name 4'-Demethylepipodophyllotoxin 9-(4,6-O-ethylidene-β-D-glucopyranoside, VP-16-213
Formula C₂₂H₂₉FO₅
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 588.56 g/mol
CAS-No 33419-42-0
Application Notes We recommend using 1000X dilutions for inducing apoptosis in Jurkat cells (e.g., Add 1 µL to 1 mL of culture medium).
However the optimal doses may vary for different cells and culture conditions.

A topoisomerase II inhibitor. A derivative of podophyllotoxin that has major activity against a number of tumors, including germ cell neoplasms, small cell lung cancer, and malignant lymphoma. Induces apoptosis in human T cells, mouse thymocytes, and HL-60 human leukemia cells

Restrictions For Research Use only
Format Liquid
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 6 months
Supplier Images
 image for Etoposide (100 mM) (ABIN412366) Etoposide (100 mM)
Product cited in: Chen, Colvin, Maier, Mirmira, Fueger: "Mitogen-inducible gene 6 triggers apoptosis and exacerbates ER stress-induced β-cell death." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 1, pp. 162-71, 2012 (PubMed).

Itamochi, Oishi, Shimada, Sato, Uegaki, Naniwa, Sato, Nonaka, Terakawa, Kigawa, Harada: "Inhibiting the mTOR pathway synergistically enhances cytotoxicity in ovarian cancer cells induced by etoposide through upregulation of c-Jun." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 14, pp. 4742-50, 2011 (PubMed).

Xia, Ma, Sun, Yang, Peng: "Retinoic acid metabolizing enzyme CYP26A1 is implicated in rat embryo implantation." in: Human reproduction (Oxford, England), Vol. 25, Issue 12, pp. 2985-98, 2010 (PubMed).

Bhola, Simon: "Determinism and divergence of apoptosis susceptibility in mammalian cells." in: Journal of cell science, Vol. 122, Issue Pt 23, pp. 4296-302, 2009 (PubMed).

Jensen, Andresen, Hansen, Skov: "Cell-surface expression of Hsp70 on hematopoietic cancer cells after inhibition of HDAC activity." in: Journal of leukocyte biology, Vol. 86, Issue 4, pp. 923-32, 2009 (PubMed).

Lin, Chen, Chang, Jan, Hsu, Wu, Fang, Tang, Chang, Lin: "Bcl-2 rescues ceramide- and etoposide-induced mitochondrial apoptosis through blockage of caspase-2 activation." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23758-65, 2005 (PubMed).