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Z-WEHD-FMK (Caspase-5 Inhibitor)

Details for Product No. ABIN412402, Supplier: Log in to see
Application
Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
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Purpose A caspase-4 inhibitor
Sequence Z-Trp-Glu(OMe)-His-Asp(OMe)-FMK
Characteristics PubChem CID: 44134610
A synthetic peptide that irreversibly inhibits caspase-5 and related caspase activity. The inhibitor is designed as a methyl ester to facilitate cell permeability. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Purity > 99 % by HPLC
Chemical Name Z-WEHD fluoromethylketone
Formula C₃₇H₄₂FN₇O₁₁
Permeability Cell-permeable
Solubility DMSO
Molecular Weight 763.78 Da
Application Notes We recommend using 1000-5000X dilutions for inhibiting caspase-5 activity in Jurkat cell culture (e.g., Add 1 µL to 1-5 mL of culture medium). The optimal doses may vary for different cells and culture conditions.
Restrictions For Research Use only
Format Liquid
Concentration 10 mM
Handling Advice Protect from light and moisture
Storage -20 °C
Expiry Date 12 months
Product cited in: Burm, Zuiderwijk-Sick, t Jong, van der Putten, Veth, Kondova, Bajramovic: "Inflammasome-induced IL-1β secretion in microglia is characterized by delayed kinetics and is only partially dependent on inflammatory caspases." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 2, pp. 678-87, 2015 (PubMed).

Gillet, Dewals, Farnir, de Leval, Vanderplasschen: "Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo." in: Cancer research, Vol. 65, Issue 20, pp. 9463-72, 2005 (PubMed).

Coletti, Yang, Marazzi, Sassoon: "TNFalpha inhibits skeletal myogenesis through a PW1-dependent pathway by recruitment of caspase pathways." in: The EMBO journal, Vol. 21, Issue 4, pp. 631-42, 2002 (PubMed).