Mouse IgG2b Isotype Control

Details for Product No. ABIN1043029
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Isotype
Synonyms IgG2b, Igh-3, gamma2b
Host
Mouse
(123), (84)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(25), (17), (17), (17), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB), Isotype Control (IsoC)
Pubmed 5 references available
Catalog no. ABIN1043029
Quantity 0.1 mg
Price
254.10 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 4 to 5 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen KLH-coupled trinitrophenol
Clone MPC-11
Isotype IgG2b
Specificity This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
No Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Characteristics The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 µg/mL ) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

It serves as a mouse IgG1 isotype control for flow cytometry experiments.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Research Area Secondary Antibodies
Application Notes The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per mL of
Incubation mixture), change the conditions of your experiment to reduce the background

Working concentrations should be determined by the investigator.
Comment

Mouse Immunoglobulin Isotype Controls

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Gupta, Wang, Vinson et al.: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, 1999 (PubMed).

Im, Tapinos, Chae et al.: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, 2006 (PubMed).

Smed-Sörensen, Moll, Cheng et al.: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, 2008 (PubMed).

Liang, Zhou, Li et al.: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." in: World journal of gastroenterology : WJG, Vol. 16, Issue 5, pp. 618-24, 2010 (PubMed).

Dewals, Vanderplasschen: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues." in: Veterinary research, Vol. 42, Issue 1, pp. 95, 2011 (PubMed).

Hosts (123), (84)
Conjugates (25), (17), (17), (17), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top