Mouse IgG Isotype Control

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Isotype
Host
Mouse
217
206
204
169
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
Antibody Type
Recombinant
Application
Isotype Control (IsoC), SDS-PAGE (SDS)
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Isotype IgG
Purification purified by chromatography
Purity > 90 % by SDS-PAGE
Background Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains (two heavy chains gamma and two light chains). Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It plays an important role in Antibody-dependent cell-mediated cytotoxicity (ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography techniques.
Synonyms: Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G
Molecular Weight 26.6 kDa (236aa)
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Comment

Concentration (detection method): 0.5 mg/mL (determined by Bradford assay)

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 20 mM Tris-HCl buffer (pH 8.0)
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C
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 image for Mouse IgG Isotype Control (ABIN1098804) Mouse IgG isotype control
Background publications Brüggemann, Teale, Clark, Bindon, Waldmann: "A matched set of rat/mouse chimeric antibodies. Identification and biological properties of rat H chain constant regions mu, gamma 1, gamma 2a, gamma 2b, gamma 2c, epsilon, and alpha." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 9, pp. 3145-50, 1989 (PubMed).