Mouse IgG2b Isotype Control

Details for Product No. ABIN1107708
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Isotype
Host
Mouse
(112), (62)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (19), (14), (12), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN1107708
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Immunogen KLH-coupled Trinitrophenol
Clone MPC-11
Isotype IgG2b
Purification Affinity Chromatography on ProteinA
Background The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/mL) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Research Area Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Comment

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/mL) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Gupta, Wang, Vinson et al.: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, 1999 (PubMed).

Im, Tapinos, Chae et al.: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, 2006 (PubMed).

Smed-Sörensen, Moll, Cheng et al.: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, 2008 (PubMed).

Validation Images
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