Mouse Immunoglobulin G3 (IgG3) isotype control (Biotin)
| Antigen |
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| Synonyms |
IgG3, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, MGC45809, DKFZp686H11213 |
| Clonality |
Monoclonal (PPV-07) |
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Host
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Reactivity
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Mouse (Murine)
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Conjugate
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Application
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Flow Cytometry (FACS)
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| Catalog no. |
ABIN125734 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
286.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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IgG3
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Isotype
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IgG3
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Clone
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PPV-07
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Description
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The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration(more than 15 µ g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control foreach specific monoclonal antibody used in a particular immunoassay. The monoclonalantibody PPV-07, generated against a plant pathogen, does not cross-react with otherspecies, and hence all the background that could be observed when working with thisantibody would be a result of general nonspecific interactions between an mouse IgG3molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
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Specificity
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This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on aplant pathogen. This antibody does not react with: Human, Porcine, Mouse, Rat, Other species Not tested (expected negative) .
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Application Notes
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The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the sameconcentration of this isotype control antibody as the recommended working concentration ofthe antigen-specific antibody. Also, when working with prediluted antibodies, dilute theisotype control to the same concentration as is the concentration of the antigen-specificantibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when Working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
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Concentration
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1 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Thereagent is free of unconjugated biotin..
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Secondary Antibodies
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Restrictions
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For Research Use only
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Alternatives for antigen "Immunoglobulin G3 (IgG3)", type "Isotype Controls"
Alternatives 
