Rabbit IgG Isotype Control

Details for Product No. ABIN1689334, Supplier: Log in to see
Isotype
Host
Rabbit
218
210
208
172
162
130
114
94
93
92
91
76
59
52
44
42
27
25
24
23
22
11
8
6
3
3
1
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Isotype Control (IsoC), Negative Control (NC)
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Immunogen The Negative Control is a recombinant rabbit monoclonal antibody against a small molecule, which is normally not present in human or animal.
Clone SP137
Isotype IgG
Specificity The product will not cross-react with human tissues when used at the recommended concentration.
Characteristics Negative Control For Rabbit IgG
Purification Purified by Protein A/G
Research Area Cardiovascular
Application Notes IHC Procedure: Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
IHC Dilution: 1:400
Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100 (2µg/mL). The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Restrictions For Research Use only
Format Liquid
Buffer purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide (concentration 200 µg/mL).
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C
Storage Comment The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Product cited in: Cano, Carmona, Ruiz-Villalba, Rojas, Chau, Wagner, Wagner, Hastie, Muñoz-Chápuli, Pérez-Pomares: "Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 3, pp. 656-61, 2016 (PubMed).

Burdelski, Menan, Tsourlakis, Kluth, Hube-Magg, Melling, Minner, Koop, Graefen, Heinzer, Wittmer, Sauter, Simon, Schlomm, Steurer, Krech: "The prognostic value of SUMO1/Sentrin specific peptidase 1 (SENP1) in prostate cancer is limited to ERG-fusion positive tumors lacking PTEN deletion." in: BMC cancer, Vol. 15, pp. 538, 2015 (PubMed).