Human IgG Isotype Control

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Isotype
Host
Human
219
209
209
171
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Clonality
Polyclonal
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Isotype IgG
Purification Protein A and peptide affinity chromatography
Background

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes WB(Assay-dependent)
ELISA(Assay-dependent)
FCM(Assay-dependent)
IHC-P(Assay-dependent)
IHC-F(Assay-dependent)
IF(IHC-P)(Assay-dependent)
IF(IHC-F)(Assay-dependent)
IF(ICC)(Assay-dependent)
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Product cited in: Pi, Long, Huang, Cheng, Zheng: "A sandwich immunoassay for detection of Aβ(1-42) based on quantum dots." in: Talanta, Vol. 146, pp. 10-5, 2015 (PubMed).

Zhou, Gan, Li, Hu, Li, Wang, Zheng: "A cost-effective sandwich electrochemiluminescence immunosensor for ultrasensitive detection of HIV-1 antibody using magnetic molecularly imprinted polymers as capture probes." in: Biosensors & bioelectronics, Vol. 54, pp. 199-206, 2014 (PubMed). Method employed by authors: ELISA (ELISA)

Gao, Wang, Wang, Han, Fu: "Amorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin." in: Analytica chimica acta, Vol. 819, pp. 102-7, 2014 (PubMed).

Li, Qiang, Wang, Vuki, Xu: "Ultrasensitive and fast fluorescent bioassay based on fluorescence enhancement of silver nanoparticles." in: The Analyst, Vol. 138, Issue 24, pp. 7376-83, 2013 (PubMed).