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HEK-293 Cells IgG3 Isotype Control

IsoC IgG3 unconjugated
Catalog No. ABIN2181279
  • Target See all IgG3 products
    IgG3
    Reactivity
    Human
    Host
    • 56
    • 8
    • 5
    HEK-293 Cells
    Application
    Isotype Control (IsoC)
    Characteristics
    Human IgG3-Fc / IGHG3 Region has a calculated MW of 31.2 kDa. The predicted N-terminus is Glu 99. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Isotype
    IgG3
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
    Buffer
    50 mM tris, 100 mM glycine, pH 7.5
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Target
    IgG3
    Abstract
    IgG3 Products
    Synonyms
    IgG3 Isotype Control, Immunoglobulin heavy constant gamma 3 Isotype Control, immunoglobulin heavy constant gamma 3 (G3m marker) Isotype Control, Ighg3 Isotype Control, IGHG3 Isotype Control
    Target Type
    Antibody
    Background
    Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
    Molecular Weight
    31.2 kDa
    UniProt
    P01860
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