Mouse IgG2a, kappa Isotype Control

Details for Product No. ABIN2656571, Supplier: Log in to see
Isotype
Host
Mouse
76
65
1
Clonality (Clone)
Monoclonal ()
Application
Isotype Control (IsoC), Western Blotting (WB)
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Clone MOPC-173
Isotype IgG2a kappa
Fragment Chain kappa
Characteristics The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Purification The immunoglobulin was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Alternative Name IgG2a kappa
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Background publications Ohno, Hattori, Yoshiki, Kakeya: "Association of epigenetic alterations in the human C7orf24 gene with the aberrant gene expression in malignant cells." in: Journal of biochemistry, Vol. 154, Issue 4, pp. 355-62, 2013 (PubMed).

Podolin, Bolognese, Carpenter, Davis, Johanson, Fox, Long, Dong, Marquis, Locastro, Terfloth, Kurali, Peterson, Smith, McQueney, Yamashita, Capper-Spudich et al.: "Inhibition of invariant chain processing, antigen-induced proliferative responses, and the development of collagen-induced arthritis and experimental autoimmune encephalomyelitis by a small molecule ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 12, pp. 7989-8003, 2008 (PubMed).

Burman, Banovic, Kuns, Clouston, Stanley, Morris, Rowe, Bofinger, Skoczylas, Raffelt, Fahy, McColl, Engwerda, McDonald, Hill: "IFNgamma differentially controls the development of idiopathic pneumonia syndrome and GVHD of the gastrointestinal tract." in: Blood, Vol. 110, Issue 3, pp. 1064-72, 2007 (PubMed).

Luckashenak, Ryszkiewicz, Ramsey, Clements: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5177-85, 2006 (PubMed).